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- W2054393067 abstract "Ethyleneimine was reacted with guanosine in aqueous medium and the products were purified by Sephadex G-10 and high-performance liquid chromatography (HPLC). Two products were identified by UV-spectroscopy: imidazole-ring opened 7-alkylguanosine and 1-alkylguanosine, accounting for 80% and 14% of all adduct radioactivity, respectively. When the incubation with ethyleneimine and guanosine or deoxyguanosine was carried out at pH 6.0 for 1 h intact 7-alkylation products were detected. The half-life of imidazole ring opening of 7-alkylguanosine was 11, 5 and 2.8 min at pH-values 7.0, 7.7 and 8.0, respectively, as measured fluorometrically at 25°C. The respective half-life of alkylated deoxyguanosine was 21 min at pH 7.7. The half-life of depurination of alkylated deoxyguanosine was 42 min at pH 6.0 and 25°C." @default.
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- W2054393067 date "1984-03-01" @default.
- W2054393067 modified "2023-09-22" @default.
- W2054393067 title "Reactions of ethyleneimine with guanosine and deoxyguanosine" @default.
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- W2054393067 doi "https://doi.org/10.1016/0009-2797(84)90138-8" @default.
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