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- W2054397415 abstract "The tyrosyl kinase and phosphatidylinositol (PI) kinase activities of human red cells have been partially purified and characterized. Although the PI kinase required detergent for solubilization, the major tyrosyl kinase of the red cell could be extracted by high salt. A very small residual activity remained associated with the membranes, however, that was solubilized with the PI kinase and copurified through an ammonium sulfate precipitation and diethylaminoethyl (DEAE) ion-exchange step gradient elution. However, the two activities were found to differ with respect to their apparent KmS for ATP and Mg2+; they showed different half-lives for temperature inactivation, possessed different relative activities in the presence of Mn2+ and Ca2+, and were separable by elution from a DEAE-Trisacryl ion exchange column using a linear NaCl gradient. The kinetic parameters of the membrane-associated tyrosyl kinase differed from those of the salt-extracted enzyme. PI kinase was not activated by pretreatment with the tyrosyl kinase p68v-ros or by addition of the phosphotyrosyl phosphatase inhibitor, vanadate, to intact membranes, and was not competitively inhibited by the tyrosyl kinase substrate poly(Glu4, Tyr). We conclude that the human red cell phosphatidylinositol and tyrosyl kinases are distinct and separate activities, and that at least two separable tyrosyl kinases are present in human erythrocytes." @default.
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- W2054397415 date "1987-04-01" @default.
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- W2054397415 title "Tyrosyl and phosphatidylinositol kinases of human erythrocyte membranes" @default.
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- W2054397415 doi "https://doi.org/10.1002/jcb.240330402" @default.
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