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- W2054399701 abstract "Abstract The aim of this study was to evaluate the forensic protocol recently developed by Qiagen for the QIAsymphony automated DNA extraction platform. Samples containing low amounts of DNA were specifically considered, since they represent the majority of samples processed in our laboratory. The analysis of simulated blood and saliva traces showed that the highest DNA yields were obtained with the maximal elution volume available for the forensic protocol, that is 200μl. Resulting DNA extracts were too diluted for successful DNA profiling and required a concentration. This additional step is time consuming and potentially increases inversion and contamination risks. The 200μl DNA extracts were concentrated to 25μl, and the DNA recovery estimated with real-time PCR as well as with the percentage of SGM Plus alleles detected. Results using our manual protocol, based on the QIAamp DNA mini kit, and the automated protocol were comparable. Further tests will be conducted to determine more precisely DNA recovery, contamination risk and PCR inhibitors removal, once a definitive procedure, allowing the concentration of DNA extracts from low yield samples, will be available for the QIAsymphony." @default.
- W2054399701 created "2016-06-24" @default.
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- W2054399701 date "2009-12-01" @default.
- W2054399701 modified "2023-09-24" @default.
- W2054399701 title "Automated DNA extraction using the QIAsymphony platform: Estimation of DNA recovery from simulated forensic stains" @default.
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- W2054399701 doi "https://doi.org/10.1016/j.fsigss.2009.07.009" @default.
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