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• W2054451352 abstract "Abstract The heavy metal polycation ruthenium red was used for identification and ultrastructural and biochemical characterization of membrane vesicles after their separation in a reorientating sucrose gradient. Simultaneous application of 0.1% (w/v) ruthenium red and 2.5% (v/v) glutaraldehyde to maize coleoptile segments resulted in a selective staining of the tonoplast. In contrast, used before fixation, ruthenium red also binds to a portion of the plasma membrane that can be isolated and characterized by molybdate-resistant phosphatase activity. Fixation with glutaraldehyde (and to a lesser amount with formaldehyde, in-vivo and in-vitro) leads to a strong inhibition of ATPases. Remaining ATP hydrolysis is rather related to the activity of unspecific phosphatase(s) measured with p-nitrophenyl phosphate." @default.
• W2054451352 created "2016-06-24" @default.
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• W2054451352 date "1991-01-01" @default.
• W2054451352 modified "2023-09-26" @default.
• W2054451352 title "Cytochemical staining of plasma membrane and tonoplast in maize coleoptiles by use of ruthenium red" @default.
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• W2054451352 doi "https://doi.org/10.1016/0168-9452(91)90049-e" @default.
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