Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054454917> ?p ?o ?g. }
- W2054454917 endingPage "3845" @default.
- W2054454917 startingPage "3838" @default.
- W2054454917 abstract "Glycan characterization of therapeutic proteins is of utmost importance due to the role of carbohydrates in protein stability, half-life, efficacy and mechanism of action. The primary assay for characterization and lot release of N-linked glycans on glycoprotein products at Genentech, Inc., is a capillary electrophoresis (CE) based assay, wherein PNGase F-released, APTS-labeled glycans are separated by CE with laser induced fluorescence (LIF) detection. With the growing number of new molecular entities in the pipeline, a fast and direct characterization approach is of increasing importance. This paper describes the development of CE−MS technology with on-line LIF detection that allows identification of major and minor glycan species (1–5% of total glycans) by providing accurate mass information. Data is presented for therapeutic rMAbs which presented previously unidentified, minor peaks during routine CE−LIF analysis. CE−LIF−MS was then used to provide accurate mass on these species, identifying CE peaks corresponding to sialylated (G1 + NANA, G2 + NANA), afucosylated (G0-GlcNAc-fucose) and low-level isomers of major APTS-labeled glycans G0, G1, G1′ and G2." @default.
- W2054454917 created "2016-06-24" @default.
- W2054454917 creator A5061381802 @default.
- W2054454917 creator A5078002121 @default.
- W2054454917 date "2008-04-22" @default.
- W2054454917 modified "2023-10-14" @default.
- W2054454917 title "On-Line CE−LIF−MS Technology for the Direct Characterization of N-Linked Glycans from Therapeutic Antibodies" @default.
- W2054454917 cites W1508509739 @default.
- W2054454917 cites W1529809181 @default.
- W2054454917 cites W1539242187 @default.
- W2054454917 cites W1549345436 @default.
- W2054454917 cites W1553087448 @default.
- W2054454917 cites W1967961992 @default.
- W2054454917 cites W1971622553 @default.
- W2054454917 cites W1973010976 @default.
- W2054454917 cites W1982911860 @default.
- W2054454917 cites W1983354426 @default.
- W2054454917 cites W1992071827 @default.
- W2054454917 cites W1993042746 @default.
- W2054454917 cites W1999139162 @default.
- W2054454917 cites W2005064823 @default.
- W2054454917 cites W2006980325 @default.
- W2054454917 cites W2009488844 @default.
- W2054454917 cites W2009729534 @default.
- W2054454917 cites W2009874301 @default.
- W2054454917 cites W2010067029 @default.
- W2054454917 cites W2016783238 @default.
- W2054454917 cites W2030932286 @default.
- W2054454917 cites W2036519286 @default.
- W2054454917 cites W2038850499 @default.
- W2054454917 cites W2049324939 @default.
- W2054454917 cites W2050633231 @default.
- W2054454917 cites W2055530646 @default.
- W2054454917 cites W2055918145 @default.
- W2054454917 cites W2074075332 @default.
- W2054454917 cites W2079579628 @default.
- W2054454917 cites W2080480217 @default.
- W2054454917 cites W2085630699 @default.
- W2054454917 cites W2111262648 @default.
- W2054454917 cites W2112410342 @default.
- W2054454917 cites W2137354517 @default.
- W2054454917 cites W2138704479 @default.
- W2054454917 cites W2164773157 @default.
- W2054454917 cites W2322897941 @default.
- W2054454917 cites W3148214213 @default.
- W2054454917 cites W4243804942 @default.
- W2054454917 cites W655139255 @default.
- W2054454917 doi "https://doi.org/10.1021/ac800152h" @default.
- W2054454917 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18426228" @default.
- W2054454917 hasPublicationYear "2008" @default.
- W2054454917 type Work @default.
- W2054454917 sameAs 2054454917 @default.
- W2054454917 citedByCount "126" @default.
- W2054454917 countsByYear W20544549172012 @default.
- W2054454917 countsByYear W20544549172013 @default.
- W2054454917 countsByYear W20544549172014 @default.
- W2054454917 countsByYear W20544549172015 @default.
- W2054454917 countsByYear W20544549172016 @default.
- W2054454917 countsByYear W20544549172017 @default.
- W2054454917 countsByYear W20544549172018 @default.
- W2054454917 countsByYear W20544549172019 @default.
- W2054454917 countsByYear W20544549172020 @default.
- W2054454917 countsByYear W20544549172021 @default.
- W2054454917 countsByYear W20544549172022 @default.
- W2054454917 countsByYear W20544549172023 @default.
- W2054454917 crossrefType "journal-article" @default.
- W2054454917 hasAuthorship W2054454917A5061381802 @default.
- W2054454917 hasAuthorship W2054454917A5078002121 @default.
- W2054454917 hasConcept C108625454 @default.
- W2054454917 hasConcept C121332964 @default.
- W2054454917 hasConcept C159654299 @default.
- W2054454917 hasConcept C171250308 @default.
- W2054454917 hasConcept C185592680 @default.
- W2054454917 hasConcept C192562407 @default.
- W2054454917 hasConcept C203014093 @default.
- W2054454917 hasConcept C204641814 @default.
- W2054454917 hasConcept C206212055 @default.
- W2054454917 hasConcept C2776817266 @default.
- W2054454917 hasConcept C2776841590 @default.
- W2054454917 hasConcept C2777313579 @default.
- W2054454917 hasConcept C2780841128 @default.
- W2054454917 hasConcept C2900643 @default.
- W2054454917 hasConcept C43617362 @default.
- W2054454917 hasConcept C55493867 @default.
- W2054454917 hasConcept C62520636 @default.
- W2054454917 hasConcept C86803240 @default.
- W2054454917 hasConcept C91881484 @default.
- W2054454917 hasConceptScore W2054454917C108625454 @default.
- W2054454917 hasConceptScore W2054454917C121332964 @default.
- W2054454917 hasConceptScore W2054454917C159654299 @default.
- W2054454917 hasConceptScore W2054454917C171250308 @default.
- W2054454917 hasConceptScore W2054454917C185592680 @default.
- W2054454917 hasConceptScore W2054454917C192562407 @default.
- W2054454917 hasConceptScore W2054454917C203014093 @default.
- W2054454917 hasConceptScore W2054454917C204641814 @default.
- W2054454917 hasConceptScore W2054454917C206212055 @default.
- W2054454917 hasConceptScore W2054454917C2776817266 @default.
- W2054454917 hasConceptScore W2054454917C2776841590 @default.