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• W2054539464 startingPage "14699" @default.
• W2054539464 abstract "To determine the dynamics of transcript extrusion from Escherichia coli RNA polymerase (RNAP), we used degradation of the RNA by RNases T1 and A in a series of consecutive elongation complexes (ECs). In intact ECs, even extremely high doses of the RNases were unable to cut the RNA closer than 14-16 nt from the 3' end. Our results prove that all of the cuts detected within the 14-nt zone are derived from the EC that is denatured during inactivation of the RNases. The protected zone monotonously translocates along the RNA after addition of new nucleotides to the transcript. The upstream region of the RNA heading toward the 5' end is cleaved and dissociated from the EC, with no effect on the stability and activity of the EC. Most of the current data suggest that an 8- to 10-nt RNA.DNA hybrid is formed in the EC. Here, we show that an 8- to 10-nt RNA obtained by truncating the RNase-generated products further with either GreB or pyrophosphate is sufficient for the high stability and activity of the EC. This result suggests that the transcript-RNAP interaction that is required for holding the EC together can be limited to the RNA region involved in the 8- to 10-nt RNA.DNA hybrid." @default.
• W2054539464 created "2016-06-24" @default.
• W2054539464 creator A5007385141 @default.
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• W2054539464 date "1998-12-08" @default.
• W2054539464 modified "2023-09-26" @default.
• W2054539464 title "Functional topography of nascent RNA in elongation intermediates of RNA polymerase" @default.
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• W2054539464 doi "https://doi.org/10.1073/pnas.95.25.14699" @default.
• W2054539464 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/24512" @default.
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