FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054579807> ?p ?o ?g. }

• W2054579807 endingPage "261" @default.
• W2054579807 startingPage "247" @default.
• W2054579807 abstract "Automated microscopy was introduced two decades ago and has become an integral part of the discovery process as a high-content screening platform with noticeable challenges in executing cell-based assays. It would be of interest to use it to screen for reversers of a transformed cell phenotype. In this report, we present data obtained from an optimized assay that identifies compounds that reverse a transformed phenotype induced in NIH-3T3 cells by expressing a novel oncogene, KP, resulting from fusion between platelet derived growth factor receptor alpha (PDGFRα) and kinase insert domain receptor (KDR), that was identified in human glioblastoma. Initial image acquisitions using multiple tiles per well were found to be insufficient as to accurately image and quantify the clusters; whole-well imaging, performed on the IN Cell Analyzer 2000, while still two-dimensional imaging, was found to accurately image and quantify clusters, due largely to the inherent variability of their size and well location. The resulting assay exhibited a Z′ value of 0.79 and a signal-to-noise ratio of 15, and it was validated against known effectors and shown to identify only PDGFRα inhibitors, and then tested in a pilot screen against a library of 58 known inhibitors identifying mostly PDGFRα inhibitors as reversers of the KP induced transformed phenotype. In conclusion, our optimized and validated assay using whole-well imaging is robust and sensitive in identifying compounds that reverse the transformed phenotype induced by KP with a broader applicability to other cell-based assays that are challenging in HTS against chemical and RNAi libraries." @default.
• W2054579807 created "2016-06-24" @default.
• W2054579807 creator A5017219096 @default.
• W2054579807 creator A5020474896 @default.
• W2054579807 creator A5048527492 @default.
• W2054579807 creator A5051905823 @default.
• W2054579807 creator A5060388998 @default.
• W2054579807 creator A5064025269 @default.
• W2054579807 creator A5076104674 @default.
• W2054579807 creator A5090616937 @default.
• W2054579807 date "2011-06-01" @default.
• W2054579807 modified "2023-09-23" @default.
• W2054579807 title "Validation of a High-Content Screening Assay Using Whole-Well Imaging of Transformed Phenotypes" @default.
• W2054579807 cites W1965254413 @default.
• W2054579807 cites W1965555958 @default.
• W2054579807 cites W1965734230 @default.
• W2054579807 cites W1970001436 @default.
• W2054579807 cites W1976858193 @default.
• W2054579807 cites W1978041336 @default.
• W2054579807 cites W1991001144 @default.
• W2054579807 cites W1994219607 @default.
• W2054579807 cites W2003470307 @default.
• W2054579807 cites W2009707750 @default.
• W2054579807 cites W2010251891 @default.
• W2054579807 cites W2010913206 @default.
• W2054579807 cites W2023447434 @default.
• W2054579807 cites W2034269086 @default.
• W2054579807 cites W2037873277 @default.
• W2054579807 cites W2055513879 @default.
• W2054579807 cites W2070262049 @default.
• W2054579807 cites W2070444157 @default.
• W2054579807 cites W2071853918 @default.
• W2054579807 cites W2078901164 @default.
• W2054579807 cites W2094454542 @default.
• W2054579807 cites W2102610315 @default.
• W2054579807 cites W2117865778 @default.
• W2054579807 cites W2127362646 @default.
• W2054579807 cites W2131403504 @default.
• W2054579807 cites W2138969252 @default.
• W2054579807 cites W2151136390 @default.
• W2054579807 cites W2156063374 @default.
• W2054579807 cites W2159072093 @default.
• W2054579807 cites W2419374772 @default.
• W2054579807 cites W4238492599 @default.
• W2054579807 doi "https://doi.org/10.1089/adt.2010.0342" @default.
• W2054579807 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3123874" @default.
• W2054579807 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21182456" @default.
• W2054579807 hasPublicationYear "2011" @default.
• W2054579807 type Work @default.
• W2054579807 sameAs 2054579807 @default.
• W2054579807 citedByCount "13" @default.
• W2054579807 countsByYear W20545798072012 @default.
• W2054579807 countsByYear W20545798072013 @default.
• W2054579807 countsByYear W20545798072014 @default.
• W2054579807 countsByYear W20545798072015 @default.
• W2054579807 countsByYear W20545798072016 @default.
• W2054579807 countsByYear W20545798072017 @default.
• W2054579807 countsByYear W20545798072018 @default.
• W2054579807 countsByYear W20545798072019 @default.
• W2054579807 countsByYear W20545798072022 @default.
• W2054579807 crossrefType "journal-article" @default.
• W2054579807 hasAuthorship W2054579807A5017219096 @default.
• W2054579807 hasAuthorship W2054579807A5020474896 @default.
• W2054579807 hasAuthorship W2054579807A5048527492 @default.
• W2054579807 hasAuthorship W2054579807A5051905823 @default.
• W2054579807 hasAuthorship W2054579807A5060388998 @default.
• W2054579807 hasAuthorship W2054579807A5064025269 @default.
• W2054579807 hasAuthorship W2054579807A5076104674 @default.
• W2054579807 hasAuthorship W2054579807A5090616937 @default.
• W2054579807 hasBestOaLocation W20545798072 @default.
• W2054579807 hasConcept C104317684 @default.
• W2054579807 hasConcept C127716648 @default.
• W2054579807 hasConcept C1491633281 @default.
• W2054579807 hasConcept C157044486 @default.
• W2054579807 hasConcept C170493617 @default.
• W2054579807 hasConcept C180361614 @default.
• W2054579807 hasConcept C2775960820 @default.
• W2054579807 hasConcept C54355233 @default.
• W2054579807 hasConcept C6555983 @default.
• W2054579807 hasConcept C70721500 @default.
• W2054579807 hasConcept C86803240 @default.
• W2054579807 hasConceptScore W2054579807C104317684 @default.
• W2054579807 hasConceptScore W2054579807C127716648 @default.
• W2054579807 hasConceptScore W2054579807C1491633281 @default.
• W2054579807 hasConceptScore W2054579807C157044486 @default.
• W2054579807 hasConceptScore W2054579807C170493617 @default.
• W2054579807 hasConceptScore W2054579807C180361614 @default.
• W2054579807 hasConceptScore W2054579807C2775960820 @default.
• W2054579807 hasConceptScore W2054579807C54355233 @default.
• W2054579807 hasConceptScore W2054579807C6555983 @default.
• W2054579807 hasConceptScore W2054579807C70721500 @default.
• W2054579807 hasConceptScore W2054579807C86803240 @default.
• W2054579807 hasIssue "3" @default.
• W2054579807 hasLocation W20545798071 @default.
• W2054579807 hasLocation W20545798072 @default.
• W2054579807 hasLocation W20545798073 @default.
• W2054579807 hasLocation W20545798074 @default.
• W2054579807 hasOpenAccess W2054579807 @default.

• next