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- W2054634291 abstract "We have studied the viability of Haemophilus spp. preserved for 5 to 12 months at -70 degrees C. The following media were used: Laboratoire de Santé Publique du Québec (LSPQ) preservation medium, trypticase soy broth with 10 degrees C (vol/vol) glycerol and 40 degrees C (vol/vol) horse serum (TSBG), and Levinthal's broth (LB) medium. Three clinical isolates of both H. influenzae and H. parainfluenzae were used. After 5 months no differences in viability were observed between strains preserved in TSBG and strains preserved in LB, but a significant loss of viability was observed in strains preserved in LSPQ medium. No significant changes in antimicrobial susceptibility were observed after 5-month storage in any medium. After 12 months, TSBG appeared to be the most suitable cryopreservation medium for the six strains tested. We conclude that TSBG represents a good medium for the maintenance of Haemophilus spp. at -70 degrees C for up to 1 year." @default.
- W2054634291 created "2016-06-24" @default.
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- W2054634291 date "2001-08-01" @default.
- W2054634291 modified "2023-09-27" @default.
- W2054634291 title "Preservation of Haemophilus influenzae and Haemophilus parainfluenzae at −70°C" @default.
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- W2054634291 doi "https://doi.org/10.1006/cryo.2001.2337" @default.
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