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• W2054655600 endingPage "73" @default.
• W2054655600 startingPage "61" @default.
• W2054655600 abstract "The aim of this work was to evaluate the genomic responses of premetamorphic sole larvae (9 days post-hatching, dph) fed diets with different lipid and triacylglycerol (TAG) content. For this purpose, two diets with high (rotifers enriched with a fish oil-based emulsion; referred to as HTAG) and low (rotifers enriched with a krill oil-based emulsion; LTAG) levels of total lipids and TAG were evaluated. Lipid class and fatty acid (FA) profiles, histological characterization of intestine, liver and pancreas and expression patterns using RNA-seq were determined. Discriminant analysis results showed that larvae could be clearly differentiated on the basis of their FA profile as a function of the diet supplied until 9dph although no difference in growth was observed. RNA-seq analysis showed that larvae fed HTAG activated coordinately the transcription of apolipoproteins (apob, apoa4, apoc2, apoe, and apobec2) and other related transcripts involved in chylomicron formation, likely to facilitate proper lipid absorption and delivery. In contrast, larvae fed LTAG showed higher mRNA levels of several pancreatic enzymes (try1a, try2, cela1, cela3, cela4, chym1, chym2, amy2a and pnlip) and appetite modulators (agrp1) and some intra- and extracellular lipases. Moreover, KEGG analysis also showed that several transcripts related to lipid metabolism and glycolysis were differentially expressed with a higher abundance in larvae fed LTAG diet. All these data suggest that early larvae were able to establish compensatory mechanisms for energy homeostasis regulating key molecules for FA and TAG biosynthesis, FA uptake and intracellular management of TAG and FA to warrant optimal growth rates." @default.
• W2054655600 created "2016-06-24" @default.
• W2054655600 creator A5009331194 @default.
• W2054655600 creator A5029999074 @default.
• W2054655600 creator A5040966031 @default.
• W2054655600 creator A5062765341 @default.
• W2054655600 creator A5082080473 @default.
• W2054655600 creator A5091616832 @default.
• W2054655600 date "2014-12-01" @default.
• W2054655600 modified "2023-10-14" @default.
• W2054655600 title "Characterization of the genomic responses in early Senegalese sole larvae fed diets with different dietary triacylglycerol and total lipids levels" @default.
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• W2054655600 doi "https://doi.org/10.1016/j.cbd.2014.09.005" @default.
• W2054655600 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25463059" @default.
• W2054655600 hasPublicationYear "2014" @default.

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