FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054656819> ?p ?o ?g. }

• W2054656819 endingPage "84" @default.
• W2054656819 startingPage "77" @default.
• W2054656819 abstract "Utilization of yeast as squalene source for commercial use is limited by relatively high production costs. The ability of Kluyveromyces lactis to grow on cheap lactose-containing diary industry wastes could improve the economy of the production process. We therefore tested the potential of this yeast for squalene production. Accumulation of squalene was induced by partial inhibition of squalene epoxidase by a specific inhibitor terbinafine. Kluyveromyces lactis cultivated on glucose and lactose media showed similar growth sensitivity to terbinafine as Saccharomyces cerevisiae. The effect of terbinafine on neutral lipid pattern was tested at concentrations with low, moderate and strong growth inhibition (2·5, 5 and 7·5 μg ml(-1) , respectively). Compared to S. cerevisiae, treatment with subinhibitory terbinafine doses had a weaker effect on steryl ester levels and total ergosterol levels in K. lactis. Quantification of squalene levels in terbinafine-treated K. lactis cells revealed high accumulation of squalene particularly in cells treated with 7·5 μg ml(-1) terbinafine in lactose medium. Terbinafine treatment stimulated the development of lipid droplets as lipid storage organelles and this effect was different in K. lactis grown on glucose or lactose media. Present report is the first attempt to utilize lactose-fermenting yeast K. lactis for production of a high-value lipid and it proves squalene epoxidase as a promising target for squalene overproduction in this yeast.Squalene is a natural substance with wide applications in food, cosmetic and pharmaceutic industries. The suitability of lactose-fermenting yeast Kluyveromyces lactis for the production of squalene was tested in the study. Partial inhibition of squalene epoxidase by specific inhibitor terbinafine resulted in high accumulation of squalene in K. lactis grown on glucose or lactose comparable to values found in terbinafine-treated Saccharomyces cerevisiae. Our results prove that K. lactis is a promising micro-organism for genetic manipulations aimed at the production of squalene on industrial waste like whey as the growth substrate." @default.
• W2054656819 created "2016-06-24" @default.
• W2054656819 creator A5040430638 @default.
• W2054656819 creator A5064069076 @default.
• W2054656819 creator A5067350692 @default.
• W2054656819 creator A5068992847 @default.
• W2054656819 creator A5091407475 @default.
• W2054656819 date "2015-05-10" @default.
• W2054656819 modified "2023-09-26" @default.
• W2054656819 title "Production of squalene by lactose-fermenting yeast <i>Kluyveromyces lactis</i> with reduced squalene epoxidase activity" @default.
• W2054656819 cites W128550577 @default.
• W2054656819 cites W1942597247 @default.
• W2054656819 cites W1967251058 @default.
• W2054656819 cites W1999771206 @default.
• W2054656819 cites W2011805571 @default.
• W2054656819 cites W2012004008 @default.
• W2054656819 cites W2014488515 @default.
• W2054656819 cites W2018917785 @default.
• W2054656819 cites W2028123717 @default.
• W2054656819 cites W2045696766 @default.
• W2054656819 cites W2059514697 @default.
• W2054656819 cites W2059790662 @default.
• W2054656819 cites W2064499338 @default.
• W2054656819 cites W2086575435 @default.
• W2054656819 cites W2094489008 @default.
• W2054656819 cites W2098671352 @default.
• W2054656819 cites W2113062198 @default.
• W2054656819 cites W2128138589 @default.
• W2054656819 cites W2158807250 @default.
• W2054656819 cites W2171339512 @default.
• W2054656819 cites W2327069864 @default.
• W2054656819 cites W2337763812 @default.
• W2054656819 doi "https://doi.org/10.1111/lam.12425" @default.
• W2054656819 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25864715" @default.
• W2054656819 hasPublicationYear "2015" @default.
• W2054656819 type Work @default.
• W2054656819 sameAs 2054656819 @default.
• W2054656819 citedByCount "17" @default.
• W2054656819 countsByYear W20546568192016 @default.
• W2054656819 countsByYear W20546568192017 @default.
• W2054656819 countsByYear W20546568192018 @default.
• W2054656819 countsByYear W20546568192019 @default.
• W2054656819 countsByYear W20546568192021 @default.
• W2054656819 countsByYear W20546568192022 @default.
• W2054656819 crossrefType "journal-article" @default.
• W2054656819 hasAuthorship W2054656819A5040430638 @default.
• W2054656819 hasAuthorship W2054656819A5064069076 @default.
• W2054656819 hasAuthorship W2054656819A5067350692 @default.
• W2054656819 hasAuthorship W2054656819A5068992847 @default.
• W2054656819 hasAuthorship W2054656819A5091407475 @default.
• W2054656819 hasConcept C100544194 @default.
• W2054656819 hasConcept C181199279 @default.
• W2054656819 hasConcept C185592680 @default.
• W2054656819 hasConcept C2776703722 @default.
• W2054656819 hasConcept C2777071850 @default.
• W2054656819 hasConcept C2777576037 @default.
• W2054656819 hasConcept C2777910421 @default.
• W2054656819 hasConcept C2778041695 @default.
• W2054656819 hasConcept C2778236110 @default.
• W2054656819 hasConcept C2778724459 @default.
• W2054656819 hasConcept C2779222958 @default.
• W2054656819 hasConcept C2779548794 @default.
• W2054656819 hasConcept C2780764520 @default.
• W2054656819 hasConcept C31903555 @default.
• W2054656819 hasConcept C553450214 @default.
• W2054656819 hasConcept C55493867 @default.
• W2054656819 hasConcept C85411794 @default.
• W2054656819 hasConcept C86803240 @default.
• W2054656819 hasConcept C89423630 @default.
• W2054656819 hasConceptScore W2054656819C100544194 @default.
• W2054656819 hasConceptScore W2054656819C181199279 @default.
• W2054656819 hasConceptScore W2054656819C185592680 @default.
• W2054656819 hasConceptScore W2054656819C2776703722 @default.
• W2054656819 hasConceptScore W2054656819C2777071850 @default.
• W2054656819 hasConceptScore W2054656819C2777576037 @default.
• W2054656819 hasConceptScore W2054656819C2777910421 @default.
• W2054656819 hasConceptScore W2054656819C2778041695 @default.
• W2054656819 hasConceptScore W2054656819C2778236110 @default.
• W2054656819 hasConceptScore W2054656819C2778724459 @default.
• W2054656819 hasConceptScore W2054656819C2779222958 @default.
• W2054656819 hasConceptScore W2054656819C2779548794 @default.
• W2054656819 hasConceptScore W2054656819C2780764520 @default.
• W2054656819 hasConceptScore W2054656819C31903555 @default.
• W2054656819 hasConceptScore W2054656819C553450214 @default.
• W2054656819 hasConceptScore W2054656819C55493867 @default.
• W2054656819 hasConceptScore W2054656819C85411794 @default.
• W2054656819 hasConceptScore W2054656819C86803240 @default.
• W2054656819 hasConceptScore W2054656819C89423630 @default.
• W2054656819 hasFunder F4320334845 @default.
• W2054656819 hasIssue "1" @default.
• W2054656819 hasLocation W20546568191 @default.
• W2054656819 hasLocation W20546568192 @default.
• W2054656819 hasOpenAccess W2054656819 @default.
• W2054656819 hasPrimaryLocation W20546568191 @default.
• W2054656819 hasRelatedWork W2002433481 @default.
• W2054656819 hasRelatedWork W2054656819 @default.
• W2054656819 hasRelatedWork W2059514697 @default.
• W2054656819 hasRelatedWork W2060006210 @default.

• next