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- W2054672927 abstract "The temperature-sensitive plasmid, pSAK3, was used in tryptophan-producing strains for tryptophanase gene disruption to block the degradation of tryptophan. Plasmid pSAK3, which consisted of the pSC103 plasmid containing a tetracycline resistant gene and a disrupted tryptophanase gene inserted by a kanamycin resistant gene, was integrated into the homologous site on the chromosome by gene recombination. Through raising the temperature of cultivation to 42 degrees C and double antibiotics screening process, the strains with disrupted tryptophanase genes could be easily selected and resulted in the generation of stable mutants that no longer exist activity of tryptophanase. Once the strain with a defected tryptophanase gene was obtained, the infection of phage P1 was applied to specifically disrupt the tryptophanase gene of any other strains. This approach also can be applied to disrupt the other targeted genes as an alternative to the conventional mutagenesis." @default.
- W2054672927 created "2016-06-24" @default.
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- W2054672927 date "1993-07-01" @default.
- W2054672927 modified "2023-10-16" @default.
- W2054672927 title "Disruption of Targeted Gene in Bacterial Chromosome by Using a Temperature-Sensitive Plasmid" @default.
- W2054672927 cites W2144634347 @default.
- W2054672927 doi "https://doi.org/10.1006/bbrc.1993.1851" @default.
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