Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054703177> ?p ?o ?g. }
- W2054703177 endingPage "1649" @default.
- W2054703177 startingPage "1649" @default.
- W2054703177 abstract "We investigated the applicability of FTIR-spectroscopy as a high throughput screening method for detection of biochemical changes in intact liver cells in bulk upon fatty acid exposure. HepG2 cells adapted to serum free (HepG2-SF) growth were exposed to four different fatty acids, three octadecenoic acids, differing in cis/trans-configuration or double bond position (oleic acid, elaidic acid and vaccenic acid) as well as palmitic acid in three days. High throughput FTIR spectroscopic measurements on dried films of intact cells showed spectra with high signal-to-noise ratio and great reproducibility. When applying principal component analysis (PCA) a clear discrimination between fatty acid exposures was observed. Higher levels of triacylglycerides were accumulated in cells exposed to elaidic acid than when exposed to the other fatty acids; the least accumulation appeared to be in cells exposed to palmitic acid. An increased absorption at ∼966 cm−1 corresponding to trans-double bond was detected upon elaidic acid exposure but not upon vaccenic acid exposure. Instead, upon vaccenic acid exposure two new absorption bands were observed at 981 and 946 cm−1 due to the presence of double bond conjugation. Raman spectroscopy on single cells, with and without treatment by vaccenic acid, confirmed the presence of conjugation. By fatty acid composition analysis, the conjugation was further specified to be conjugated linoleic acid (CLA) isomers. Thus, instead of being preserved as a monounsaturated fatty acid, vaccenic acid was converted into CLA in HepG2 cells. The results demonstrate the applicability of high-throughput FTIR spectroscopy as an explorative method in in vitro systems from which biologically relevant hypotheses can be generated and further investigated." @default.
- W2054703177 created "2016-06-24" @default.
- W2054703177 creator A5012062113 @default.
- W2054703177 creator A5014987374 @default.
- W2054703177 creator A5017506005 @default.
- W2054703177 creator A5026691118 @default.
- W2054703177 creator A5028184221 @default.
- W2054703177 creator A5049085401 @default.
- W2054703177 creator A5072093489 @default.
- W2054703177 creator A5072420987 @default.
- W2054703177 date "2011-01-01" @default.
- W2054703177 modified "2023-09-26" @default.
- W2054703177 title "Monitoring cellular responses upon fatty acid exposure by Fourier transform infrared spectroscopy and Raman spectroscopy" @default.
- W2054703177 cites W1783593014 @default.
- W2054703177 cites W1879047861 @default.
- W2054703177 cites W1942808992 @default.
- W2054703177 cites W1966917335 @default.
- W2054703177 cites W1975522965 @default.
- W2054703177 cites W1976280700 @default.
- W2054703177 cites W1984559101 @default.
- W2054703177 cites W1987459669 @default.
- W2054703177 cites W1988992512 @default.
- W2054703177 cites W1992369020 @default.
- W2054703177 cites W1993023311 @default.
- W2054703177 cites W1995756653 @default.
- W2054703177 cites W2002553816 @default.
- W2054703177 cites W2007742952 @default.
- W2054703177 cites W2016298359 @default.
- W2054703177 cites W2020060179 @default.
- W2054703177 cites W2031477530 @default.
- W2054703177 cites W2035244841 @default.
- W2054703177 cites W2047033661 @default.
- W2054703177 cites W2048187570 @default.
- W2054703177 cites W2049629703 @default.
- W2054703177 cites W2050660312 @default.
- W2054703177 cites W2053477061 @default.
- W2054703177 cites W2055721275 @default.
- W2054703177 cites W2055794415 @default.
- W2054703177 cites W2057685086 @default.
- W2054703177 cites W2065262686 @default.
- W2054703177 cites W2067717363 @default.
- W2054703177 cites W2068657486 @default.
- W2054703177 cites W2072849924 @default.
- W2054703177 cites W2073085129 @default.
- W2054703177 cites W2075629601 @default.
- W2054703177 cites W2088762201 @default.
- W2054703177 cites W2100692021 @default.
- W2054703177 cites W2108352389 @default.
- W2054703177 cites W2109606373 @default.
- W2054703177 cites W2136055419 @default.
- W2054703177 cites W2144535719 @default.
- W2054703177 cites W2152311269 @default.
- W2054703177 cites W2154830597 @default.
- W2054703177 cites W2170156685 @default.
- W2054703177 cites W2316325669 @default.
- W2054703177 doi "https://doi.org/10.1039/c0an00916d" @default.
- W2054703177 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21347493" @default.
- W2054703177 hasPublicationYear "2011" @default.
- W2054703177 type Work @default.
- W2054703177 sameAs 2054703177 @default.
- W2054703177 citedByCount "28" @default.
- W2054703177 countsByYear W20547031772012 @default.
- W2054703177 countsByYear W20547031772013 @default.
- W2054703177 countsByYear W20547031772014 @default.
- W2054703177 countsByYear W20547031772015 @default.
- W2054703177 countsByYear W20547031772016 @default.
- W2054703177 countsByYear W20547031772017 @default.
- W2054703177 countsByYear W20547031772018 @default.
- W2054703177 countsByYear W20547031772019 @default.
- W2054703177 countsByYear W20547031772020 @default.
- W2054703177 countsByYear W20547031772021 @default.
- W2054703177 countsByYear W20547031772022 @default.
- W2054703177 countsByYear W20547031772023 @default.
- W2054703177 crossrefType "journal-article" @default.
- W2054703177 hasAuthorship W2054703177A5012062113 @default.
- W2054703177 hasAuthorship W2054703177A5014987374 @default.
- W2054703177 hasAuthorship W2054703177A5017506005 @default.
- W2054703177 hasAuthorship W2054703177A5026691118 @default.
- W2054703177 hasAuthorship W2054703177A5028184221 @default.
- W2054703177 hasAuthorship W2054703177A5049085401 @default.
- W2054703177 hasAuthorship W2054703177A5072093489 @default.
- W2054703177 hasAuthorship W2054703177A5072420987 @default.
- W2054703177 hasConcept C120665830 @default.
- W2054703177 hasConcept C121332964 @default.
- W2054703177 hasConcept C125287762 @default.
- W2054703177 hasConcept C153642686 @default.
- W2054703177 hasConcept C159985019 @default.
- W2054703177 hasConcept C160892712 @default.
- W2054703177 hasConcept C178790620 @default.
- W2054703177 hasConcept C185592680 @default.
- W2054703177 hasConcept C192562407 @default.
- W2054703177 hasConcept C201268389 @default.
- W2054703177 hasConcept C2777921831 @default.
- W2054703177 hasConcept C2778738395 @default.
- W2054703177 hasConcept C2779780233 @default.
- W2054703177 hasConcept C2779936771 @default.
- W2054703177 hasConcept C2780809830 @default.
- W2054703177 hasConcept C2781403372 @default.