Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054722692> ?p ?o ?g. }
- W2054722692 endingPage "e1375" @default.
- W2054722692 startingPage "e1375" @default.
- W2054722692 abstract "Patients with Chronic Chagas' Heart Disease possess high levels of antibodies against the carboxyl-terminal end of the ribosomal P2ß protein of Trypanosoma cruzi (TcP2ß). These antibodies, as well as the murine monoclonal antibody (mAb) 17.2, recognize the last 13 amino acids of TcP2ß (called the R13 epitope: EEEDDDMGFGLFD) and are able to cross-react with, and stimulate, the ß1 adrenergic receptor (ß1-AR). Indeed, the mAb 17.2 was able to specifically detect human β1-AR, stably transfected into HEK cells, by flow cytometry and to induce repolarisation abnormalities and first degree atrioventricular conduction block after passive transfer to naïve mice. To study the structural basis of this cross-reactivity, we determined the crystal structure of the Fab region of the mAb 17.2 alone at 2.31 Å resolution and in complex with the R13 peptide at 1.89 Å resolution. We identified as key contact residues on R13 peptide Glu3, Asp6 and Phe9 as was previously shown by alanine scanning. Additionally, we generated a model of human β1-AR to elucidate the interaction with anti-R13 antibodies. These data provide an understanding of the molecular basis of cross-reactive antibodies induced by chronic infection with Trypanosoma cruzi." @default.
- W2054722692 created "2016-06-24" @default.
- W2054722692 creator A5010512834 @default.
- W2054722692 creator A5013640385 @default.
- W2054722692 creator A5019910670 @default.
- W2054722692 creator A5028202360 @default.
- W2054722692 creator A5033752359 @default.
- W2054722692 creator A5035164915 @default.
- W2054722692 creator A5068519206 @default.
- W2054722692 creator A5071410670 @default.
- W2054722692 date "2011-11-01" @default.
- W2054722692 modified "2023-10-14" @default.
- W2054722692 title "Crystal Structure of the Complex mAb 17.2 and the C-Terminal Region of Trypanosoma cruzi P2β Protein: Implications in Cross-Reactivity" @default.
- W2054722692 cites W1484382785 @default.
- W2054722692 cites W1504180470 @default.
- W2054722692 cites W1537505047 @default.
- W2054722692 cites W1539796472 @default.
- W2054722692 cites W1556580063 @default.
- W2054722692 cites W1768923368 @default.
- W2054722692 cites W1908561802 @default.
- W2054722692 cites W1966430295 @default.
- W2054722692 cites W1970287936 @default.
- W2054722692 cites W1979661926 @default.
- W2054722692 cites W1979885599 @default.
- W2054722692 cites W1994602811 @default.
- W2054722692 cites W1999549385 @default.
- W2054722692 cites W2006661094 @default.
- W2054722692 cites W2008261416 @default.
- W2054722692 cites W2009212628 @default.
- W2054722692 cites W2013771584 @default.
- W2054722692 cites W2018003299 @default.
- W2054722692 cites W2019286099 @default.
- W2054722692 cites W2027071449 @default.
- W2054722692 cites W2030929959 @default.
- W2054722692 cites W2039384269 @default.
- W2054722692 cites W2039740436 @default.
- W2054722692 cites W2054956433 @default.
- W2054722692 cites W2056034415 @default.
- W2054722692 cites W2061766263 @default.
- W2054722692 cites W2066672205 @default.
- W2054722692 cites W2080476827 @default.
- W2054722692 cites W2087840775 @default.
- W2054722692 cites W2101555566 @default.
- W2054722692 cites W2102707929 @default.
- W2054722692 cites W2111718153 @default.
- W2054722692 cites W2116153018 @default.
- W2054722692 cites W2118024111 @default.
- W2054722692 cites W2123565103 @default.
- W2054722692 cites W2127739127 @default.
- W2054722692 cites W2130672098 @default.
- W2054722692 cites W2140035123 @default.
- W2054722692 cites W2144081223 @default.
- W2054722692 cites W2158907014 @default.
- W2054722692 cites W2163206873 @default.
- W2054722692 cites W2165807417 @default.
- W2054722692 cites W2170304291 @default.
- W2054722692 cites W2171131376 @default.
- W2054722692 cites W2406801630 @default.
- W2054722692 cites W4210980497 @default.
- W2054722692 cites W4302080484 @default.
- W2054722692 doi "https://doi.org/10.1371/journal.pntd.0001375" @default.
- W2054722692 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3206007" @default.
- W2054722692 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22069505" @default.
- W2054722692 hasPublicationYear "2011" @default.
- W2054722692 type Work @default.
- W2054722692 sameAs 2054722692 @default.
- W2054722692 citedByCount "6" @default.
- W2054722692 countsByYear W20547226922012 @default.
- W2054722692 countsByYear W20547226922013 @default.
- W2054722692 countsByYear W20547226922014 @default.
- W2054722692 countsByYear W20547226922015 @default.
- W2054722692 crossrefType "journal-article" @default.
- W2054722692 hasAuthorship W2054722692A5010512834 @default.
- W2054722692 hasAuthorship W2054722692A5013640385 @default.
- W2054722692 hasAuthorship W2054722692A5019910670 @default.
- W2054722692 hasAuthorship W2054722692A5028202360 @default.
- W2054722692 hasAuthorship W2054722692A5033752359 @default.
- W2054722692 hasAuthorship W2054722692A5035164915 @default.
- W2054722692 hasAuthorship W2054722692A5068519206 @default.
- W2054722692 hasAuthorship W2054722692A5071410670 @default.
- W2054722692 hasBestOaLocation W20547226921 @default.
- W2054722692 hasConcept C104317684 @default.
- W2054722692 hasConcept C112418997 @default.
- W2054722692 hasConcept C136764020 @default.
- W2054722692 hasConcept C153911025 @default.
- W2054722692 hasConcept C159047783 @default.
- W2054722692 hasConcept C159654299 @default.
- W2054722692 hasConcept C16318435 @default.
- W2054722692 hasConcept C185592680 @default.
- W2054722692 hasConcept C195616568 @default.
- W2054722692 hasConcept C203014093 @default.
- W2054722692 hasConcept C2777625466 @default.
- W2054722692 hasConcept C2779281246 @default.
- W2054722692 hasConcept C2780227090 @default.
- W2054722692 hasConcept C2781241564 @default.
- W2054722692 hasConcept C41008148 @default.
- W2054722692 hasConcept C501734568 @default.
- W2054722692 hasConcept C542903549 @default.