FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054799076> ?p ?o ?g. }

• W2054799076 endingPage "e3264" @default.
• W2054799076 startingPage "e3264" @default.
• W2054799076 abstract "Conditional gene targeting using the Cre-loxp system is a well established technique in numerous in vitro and in vivo systems. Ligand regulated forms of Cre have been increasingly used in these applications in order to gain temporal and spatial control over conditional targeting. The tamoxifen-regulated Cre variant mer-Cre-mer (mCrem) is widely utilized because of its reputation for tight regulation in the absence of its tamoxifen ligand. In the DT40 chicken B cell line, we generated an mCrem-based reversible switch for conditional regulation of a transgene, and in contrast with previous work, observed significant constitutive activity of mCrem. This prompted us to use our system for analysis of the parameters governing tamoxifen-regulated mCrem recombination of a genomic target. We find that robust mCrem expression correlates with a high level of tamoxifen-independent Cre activity, while clones expressing mCrem at the limit of western blot detection exhibit extremely tight regulation. We also observe time and dose-dependent effects on mCrem activity which suggest limitations on the use of conditional targeting approaches for applications which require tight temporal coordination of Cre action within a cell population." @default.
• W2054799076 created "2016-06-24" @default.
• W2054799076 creator A5013281585 @default.
• W2054799076 creator A5063879916 @default.
• W2054799076 date "2008-09-23" @default.
• W2054799076 modified "2023-09-26" @default.
• W2054799076 title "Characterization of Parameters Required for Effective Use of Tamoxifen-Regulated Recombination" @default.
• W2054799076 cites W105069158 @default.
• W2054799076 cites W106659398 @default.
• W2054799076 cites W121654528 @default.
• W2054799076 cites W1523950660 @default.
• W2054799076 cites W1538926863 @default.
• W2054799076 cites W1559892880 @default.
• W2054799076 cites W1967238067 @default.
• W2054799076 cites W1967528766 @default.
• W2054799076 cites W1970669095 @default.
• W2054799076 cites W1975734011 @default.
• W2054799076 cites W1983316915 @default.
• W2054799076 cites W1983804855 @default.
• W2054799076 cites W1984300343 @default.
• W2054799076 cites W2002669389 @default.
• W2054799076 cites W2009527263 @default.
• W2054799076 cites W2018258700 @default.
• W2054799076 cites W2037872087 @default.
• W2054799076 cites W2042742511 @default.
• W2054799076 cites W2042796276 @default.
• W2054799076 cites W2043772793 @default.
• W2054799076 cites W2058823301 @default.
• W2054799076 cites W2068681382 @default.
• W2054799076 cites W2069259673 @default.
• W2054799076 cites W2069795464 @default.
• W2054799076 cites W2070002807 @default.
• W2054799076 cites W2073279019 @default.
• W2054799076 cites W2076023748 @default.
• W2054799076 cites W2089518215 @default.
• W2054799076 cites W2103035251 @default.
• W2054799076 cites W2103972911 @default.
• W2054799076 cites W2132916293 @default.
• W2054799076 cites W2134208169 @default.
• W2054799076 cites W2134918940 @default.
• W2054799076 cites W2146150145 @default.
• W2054799076 cites W2209738096 @default.
• W2054799076 cites W4232865795 @default.
• W2054799076 cites W7308710 @default.
• W2054799076 doi "https://doi.org/10.1371/journal.pone.0003264" @default.
• W2054799076 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2533124" @default.
• W2054799076 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18810268" @default.
• W2054799076 hasPublicationYear "2008" @default.
• W2054799076 type Work @default.
• W2054799076 sameAs 2054799076 @default.
• W2054799076 citedByCount "12" @default.
• W2054799076 countsByYear W20547990762012 @default.
• W2054799076 countsByYear W20547990762015 @default.
• W2054799076 countsByYear W20547990762016 @default.
• W2054799076 countsByYear W20547990762017 @default.
• W2054799076 countsByYear W20547990762018 @default.
• W2054799076 countsByYear W20547990762021 @default.
• W2054799076 countsByYear W20547990762022 @default.
• W2054799076 crossrefType "journal-article" @default.
• W2054799076 hasAuthorship W2054799076A5013281585 @default.
• W2054799076 hasAuthorship W2054799076A5063879916 @default.
• W2054799076 hasBestOaLocation W20547990761 @default.
• W2054799076 hasConcept C102230213 @default.
• W2054799076 hasConcept C104317684 @default.
• W2054799076 hasConcept C121608353 @default.
• W2054799076 hasConcept C2777176818 @default.
• W2054799076 hasConcept C2908647359 @default.
• W2054799076 hasConcept C530470458 @default.
• W2054799076 hasConcept C54355233 @default.
• W2054799076 hasConcept C70721500 @default.
• W2054799076 hasConcept C71924100 @default.
• W2054799076 hasConcept C86803240 @default.
• W2054799076 hasConcept C95444343 @default.
• W2054799076 hasConcept C99454951 @default.
• W2054799076 hasConceptScore W2054799076C102230213 @default.
• W2054799076 hasConceptScore W2054799076C104317684 @default.
• W2054799076 hasConceptScore W2054799076C121608353 @default.
• W2054799076 hasConceptScore W2054799076C2777176818 @default.
• W2054799076 hasConceptScore W2054799076C2908647359 @default.
• W2054799076 hasConceptScore W2054799076C530470458 @default.
• W2054799076 hasConceptScore W2054799076C54355233 @default.
• W2054799076 hasConceptScore W2054799076C70721500 @default.
• W2054799076 hasConceptScore W2054799076C71924100 @default.
• W2054799076 hasConceptScore W2054799076C86803240 @default.
• W2054799076 hasConceptScore W2054799076C95444343 @default.
• W2054799076 hasConceptScore W2054799076C99454951 @default.
• W2054799076 hasIssue "9" @default.
• W2054799076 hasLocation W20547990761 @default.
• W2054799076 hasLocation W20547990762 @default.
• W2054799076 hasLocation W20547990763 @default.
• W2054799076 hasOpenAccess W2054799076 @default.
• W2054799076 hasPrimaryLocation W20547990761 @default.
• W2054799076 hasRelatedWork W1828691184 @default.
• W2054799076 hasRelatedWork W1903732681 @default.
• W2054799076 hasRelatedWork W1991523530 @default.
• W2054799076 hasRelatedWork W2002128513 @default.
• W2054799076 hasRelatedWork W2020824267 @default.
• W2054799076 hasRelatedWork W2031436818 @default.

• next