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• W2054833110 abstract "Somatic embryogenesis was obtained from 14 Asparagus officinalis L. genotypes by in vitro culture of different explant types such as cladophylls, apices and isolated mesophyll cells. Explants first induced on a culture medium containing growth regulators produced calli and developed few somatic embryos when transferred on a hormone-free medium. During this development phase, we isolated a new type of friable tissues that produced abundant somatic embryos. The frequencies of emergence of these highly embryogenic tissues were estimated between 10−5 and 10−6. These tissue were habituated; being established as long-term embryogenic lines by repeated subcultures on hormone-free medium, continuously they produced numerous somatic embryos, some of them converting to whole plants. Histological data showed these long-term embryogenic lines to grow by recurrent embryogenesis arising, from single epidermal cells of preexisting embryos." @default.
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• W2054833110 date "1994-08-01" @default.
• W2054833110 modified "2023-10-16" @default.
• W2054833110 title "Isolation and Characterization of Long-term Embryogenic Lines in Asparagus officinalis L." @default.
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• W2054833110 doi "https://doi.org/10.1016/s0176-1617(11)80543-2" @default.
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