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• W2054850168 abstract "To investigate the mitochondrial function and caspase activity in mouse embryos after human oviductal cell coculture.Experimental laboratory study.University gynecology unit.MF-1 (female); BALB/c (male) mice.Mouse embryos were cocultured with human oviductal cells.Mitochondrial transmembrane potential (Delta psi(m)) and caspase activity.Compared to embryos after coculture in Chatot-Ziomek-Bavister (CZB) medium supplemented with 0.5 mg/mL of BSA (CZB), Delta psi m of embryos cultured in CZB was significantly lower at the two-cell (CZB, 2.04 +/- 0.412; coculture, 4.34 +/- 0.563) and morula (CZB, 6.06 +/- 0.548; coculture, 7.12 +/- 0.568) stages. Cocultured embryos and in vivo developed embryos had comparable Delta psi m. Caspase activity was not detected in unfragmented cleavage-stage embryos and morula developed in vivo. In vitro cultured morula possessed caspase activity. The activity was significantly reduced in the cocultured morula.Human oviductal cells maintained the mitochondria function in terms of mitochondrial transmembrane potential and decreased the caspase activity to improve the development of mouse embryo." @default.
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• W2054850168 date "2003-07-01" @default.
• W2054850168 modified "2023-10-09" @default.
• W2054850168 title "Coculture of human oviductal cells maintains mitochondrial function and decreases caspase activity of cleavage-stage mouse embryos" @default.
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• W2054850168 doi "https://doi.org/10.1016/s0015-0282(03)00570-3" @default.
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