Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054886233> ?p ?o ?g. }
- W2054886233 endingPage "2073" @default.
- W2054886233 startingPage "2063" @default.
- W2054886233 abstract "The development of techniques allowing the growth of primordial follicles to mature follicles in vitro has much potential for both reproductive medicine and developmental research. However, human primordial and preantral follicles fail to grow after isolation from the surrounding ovarian stroma. Granulosa cells, which normally undergo apoptosis after ovulation, contain a subpopulation of ovarian follicular cells remaining viable in vitro over prolonged periods when cultured in the presence of leukemia-inhibiting factor. However, when cultured as monolayers, they progressively lose all their characteristics, such as follicle-stimulating hormone receptor and cytochrome P450-aromatase. Here, we describe a three-dimensional culture system containing type I collagen, which, together with leukemia-inhibiting factor, allowed the survival and growth of a subpopulation of granulosa cells isolated from mature ovarian follicles and supported them to proliferate into spherical structures exhibiting steroidogenic capacity, as demonstrated by P450-aromatase and 3β-hydroxysteroid dehydrogenase. After transplantation into the ovaries of immunodeficient mice, these cells became localized preferentially within antral follicles and the prolonged expression of follicle-stimulating hormone receptor was confirmed as well. With this optimization of the culture conditions, an environment was created, which acts as a niche closely mimicking the development of early ovarian follicles in vitro." @default.
- W2054886233 created "2016-06-24" @default.
- W2054886233 creator A5007014214 @default.
- W2054886233 creator A5033435363 @default.
- W2054886233 creator A5043355162 @default.
- W2054886233 creator A5046492899 @default.
- W2054886233 creator A5050261591 @default.
- W2054886233 creator A5052921186 @default.
- W2054886233 creator A5064633149 @default.
- W2054886233 date "2010-06-01" @default.
- W2054886233 modified "2023-09-25" @default.
- W2054886233 title "A Novel Three-Dimensional Culture System Allows Prolonged Culture of Functional Human Granulosa Cells and Mimics the Ovarian Environment" @default.
- W2054886233 cites W1972886658 @default.
- W2054886233 cites W1976989509 @default.
- W2054886233 cites W2005211911 @default.
- W2054886233 cites W2005233988 @default.
- W2054886233 cites W2006192685 @default.
- W2054886233 cites W2019108353 @default.
- W2054886233 cites W2028067787 @default.
- W2054886233 cites W2030873226 @default.
- W2054886233 cites W2037965606 @default.
- W2054886233 cites W2039550497 @default.
- W2054886233 cites W2040511639 @default.
- W2054886233 cites W2044581876 @default.
- W2054886233 cites W2046247568 @default.
- W2054886233 cites W2048120373 @default.
- W2054886233 cites W2054325204 @default.
- W2054886233 cites W2059921402 @default.
- W2054886233 cites W2062953809 @default.
- W2054886233 cites W2066617970 @default.
- W2054886233 cites W2077075203 @default.
- W2054886233 cites W2081104479 @default.
- W2054886233 cites W2084512982 @default.
- W2054886233 cites W2088780763 @default.
- W2054886233 cites W2089206964 @default.
- W2054886233 cites W2104398852 @default.
- W2054886233 cites W2105688914 @default.
- W2054886233 cites W2122685595 @default.
- W2054886233 cites W2122713961 @default.
- W2054886233 cites W2126113852 @default.
- W2054886233 cites W2140660246 @default.
- W2054886233 cites W2142928349 @default.
- W2054886233 cites W2148973603 @default.
- W2054886233 cites W2268478864 @default.
- W2054886233 cites W4233516676 @default.
- W2054886233 cites W4245403685 @default.
- W2054886233 doi "https://doi.org/10.1089/ten.tea.2009.0684" @default.
- W2054886233 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20109057" @default.
- W2054886233 hasPublicationYear "2010" @default.
- W2054886233 type Work @default.
- W2054886233 sameAs 2054886233 @default.
- W2054886233 citedByCount "26" @default.
- W2054886233 countsByYear W20548862332012 @default.
- W2054886233 countsByYear W20548862332013 @default.
- W2054886233 countsByYear W20548862332014 @default.
- W2054886233 countsByYear W20548862332015 @default.
- W2054886233 countsByYear W20548862332016 @default.
- W2054886233 countsByYear W20548862332017 @default.
- W2054886233 countsByYear W20548862332018 @default.
- W2054886233 countsByYear W20548862332019 @default.
- W2054886233 countsByYear W20548862332020 @default.
- W2054886233 countsByYear W20548862332021 @default.
- W2054886233 countsByYear W20548862332022 @default.
- W2054886233 countsByYear W20548862332023 @default.
- W2054886233 crossrefType "journal-article" @default.
- W2054886233 hasAuthorship W2054886233A5007014214 @default.
- W2054886233 hasAuthorship W2054886233A5033435363 @default.
- W2054886233 hasAuthorship W2054886233A5043355162 @default.
- W2054886233 hasAuthorship W2054886233A5046492899 @default.
- W2054886233 hasAuthorship W2054886233A5050261591 @default.
- W2054886233 hasAuthorship W2054886233A5052921186 @default.
- W2054886233 hasAuthorship W2054886233A5064633149 @default.
- W2054886233 hasBestOaLocation W20548862332 @default.
- W2054886233 hasConcept C121608353 @default.
- W2054886233 hasConcept C126322002 @default.
- W2054886233 hasConcept C134018914 @default.
- W2054886233 hasConcept C142773365 @default.
- W2054886233 hasConcept C179933525 @default.
- W2054886233 hasConcept C187785154 @default.
- W2054886233 hasConcept C196843134 @default.
- W2054886233 hasConcept C202751555 @default.
- W2054886233 hasConcept C2776166826 @default.
- W2054886233 hasConcept C2777702977 @default.
- W2054886233 hasConcept C2778324911 @default.
- W2054886233 hasConcept C2780536345 @default.
- W2054886233 hasConcept C530470458 @default.
- W2054886233 hasConcept C5512455 @default.
- W2054886233 hasConcept C55493867 @default.
- W2054886233 hasConcept C71315377 @default.
- W2054886233 hasConcept C71924100 @default.
- W2054886233 hasConcept C86803240 @default.
- W2054886233 hasConcept C95444343 @default.
- W2054886233 hasConceptScore W2054886233C121608353 @default.
- W2054886233 hasConceptScore W2054886233C126322002 @default.
- W2054886233 hasConceptScore W2054886233C134018914 @default.
- W2054886233 hasConceptScore W2054886233C142773365 @default.
- W2054886233 hasConceptScore W2054886233C179933525 @default.
- W2054886233 hasConceptScore W2054886233C187785154 @default.