Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054977793> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2054977793 abstract "α-Tropomyosin (αTm) is the predominant tropomyosin isoform in adult human heart and constitutes a major component in Ca2+-regulated systolic contraction of cardiac muscle. The familial hypertrophic cardiomyopathy (FHC)-related αTm E180G mutant is associated with decreased thermal stability and enhanced Ca2+-sensitivity in functional assays. A mechanistic relationship between the E180G missense mutation and functional changes has not been established. We present here the first direct probe images of WT human cardiac and E180G mutant αTm by atomic force microscopy, and demonstrate that the mutant is more flexible than WT. Single molecules of bacterially-expressed human cardiac αTm were imaged on poly-lysine coated mica and their contours were analyzed. Analysis of tangent correlation along molecular contours yielded values of persistence length (Lp), which showed 27% increase in flexibility of the E180G mutant. Increased flexibility of the mutant was confirmed by shorter mean end-to-end length and fitting end-to-end length distributions to the worm-like chain model. Corresponding to increased flexibility, it is expected that less mechanical moment, and hence a lesser extent of Ca2+-induced conformational change of troponin, are required to perturb αTm to initiate thin filament activation during systole, thus explaining enhanced Ca2+-sensitivity of function. Hypersensitivity to Ca2+ could overwork cardiac muscle resulting in hypertrophy in FHC. Support: NIH and American Heart Association." @default.
- W2054977793 created "2016-06-24" @default.
- W2054977793 creator A5019020936 @default.
- W2054977793 creator A5023205543 @default.
- W2054977793 creator A5085752407 @default.
- W2054977793 date "2011-02-01" @default.
- W2054977793 modified "2023-09-26" @default.
- W2054977793 title "Flexibility Change in Human Cardiac α-Tropomyosin E180G Mutant: Possible Link to Cardiac Hypertrophy" @default.
- W2054977793 doi "https://doi.org/10.1016/j.bpj.2010.12.3384" @default.
- W2054977793 hasPublicationYear "2011" @default.
- W2054977793 type Work @default.
- W2054977793 sameAs 2054977793 @default.
- W2054977793 citedByCount "1" @default.
- W2054977793 countsByYear W20549777932012 @default.
- W2054977793 crossrefType "journal-article" @default.
- W2054977793 hasAuthorship W2054977793A5019020936 @default.
- W2054977793 hasAuthorship W2054977793A5023205543 @default.
- W2054977793 hasAuthorship W2054977793A5085752407 @default.
- W2054977793 hasBestOaLocation W20549777931 @default.
- W2054977793 hasConcept C104317684 @default.
- W2054977793 hasConcept C105702510 @default.
- W2054977793 hasConcept C12554922 @default.
- W2054977793 hasConcept C125705527 @default.
- W2054977793 hasConcept C126322002 @default.
- W2054977793 hasConcept C143065580 @default.
- W2054977793 hasConcept C185592680 @default.
- W2054977793 hasConcept C205875245 @default.
- W2054977793 hasConcept C2778996579 @default.
- W2054977793 hasConcept C2780185194 @default.
- W2054977793 hasConcept C501734568 @default.
- W2054977793 hasConcept C53345823 @default.
- W2054977793 hasConcept C55493867 @default.
- W2054977793 hasConcept C71924100 @default.
- W2054977793 hasConcept C75563809 @default.
- W2054977793 hasConcept C86803240 @default.
- W2054977793 hasConceptScore W2054977793C104317684 @default.
- W2054977793 hasConceptScore W2054977793C105702510 @default.
- W2054977793 hasConceptScore W2054977793C12554922 @default.
- W2054977793 hasConceptScore W2054977793C125705527 @default.
- W2054977793 hasConceptScore W2054977793C126322002 @default.
- W2054977793 hasConceptScore W2054977793C143065580 @default.
- W2054977793 hasConceptScore W2054977793C185592680 @default.
- W2054977793 hasConceptScore W2054977793C205875245 @default.
- W2054977793 hasConceptScore W2054977793C2778996579 @default.
- W2054977793 hasConceptScore W2054977793C2780185194 @default.
- W2054977793 hasConceptScore W2054977793C501734568 @default.
- W2054977793 hasConceptScore W2054977793C53345823 @default.
- W2054977793 hasConceptScore W2054977793C55493867 @default.
- W2054977793 hasConceptScore W2054977793C71924100 @default.
- W2054977793 hasConceptScore W2054977793C75563809 @default.
- W2054977793 hasConceptScore W2054977793C86803240 @default.
- W2054977793 hasLocation W20549777931 @default.
- W2054977793 hasOpenAccess W2054977793 @default.
- W2054977793 hasPrimaryLocation W20549777931 @default.
- W2054977793 hasRelatedWork W1972454913 @default.
- W2054977793 hasRelatedWork W1998667290 @default.
- W2054977793 hasRelatedWork W2044360039 @default.
- W2054977793 hasRelatedWork W2054808162 @default.
- W2054977793 hasRelatedWork W2075077936 @default.
- W2054977793 hasRelatedWork W2076341770 @default.
- W2054977793 hasRelatedWork W2076550365 @default.
- W2054977793 hasRelatedWork W2078780525 @default.
- W2054977793 hasRelatedWork W2083986735 @default.
- W2054977793 hasRelatedWork W2143276030 @default.
- W2054977793 hasRelatedWork W2416935433 @default.
- W2054977793 hasRelatedWork W2501349141 @default.
- W2054977793 hasRelatedWork W2529236488 @default.
- W2054977793 hasRelatedWork W2584979448 @default.
- W2054977793 hasRelatedWork W2795943094 @default.
- W2054977793 hasRelatedWork W3090804336 @default.
- W2054977793 hasRelatedWork W3092937848 @default.
- W2054977793 hasRelatedWork W3129556747 @default.
- W2054977793 hasRelatedWork W3145534094 @default.
- W2054977793 hasRelatedWork W3184131855 @default.
- W2054977793 isParatext "false" @default.
- W2054977793 isRetracted "false" @default.
- W2054977793 magId "2054977793" @default.
- W2054977793 workType "article" @default.