FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054981823> ?p ?o ?g. }

• W2054981823 endingPage "e0109469" @default.
• W2054981823 startingPage "e0109469" @default.
• W2054981823 abstract "Here, we explore the regulatory mechanism of lipid metabolic signaling pathways and related genes during differentiation of male germ cells in chickens, with the hope that better understanding of these pathways may improve in vitro induction. Fluorescence-activated cell sorting was used to obtain highly purified cultures of embryonic stem cells (ESCs), primitive germ cells (PGCs), and spermatogonial stem cells (SSCs). The total RNA was then extracted from each type of cell. High-throughput analysis methods (RNA-seq) were used to sequence the transcriptome of these cells. Gene Ontology (GO) analysis and the KEGG database were used to identify lipid metabolism pathways and related genes. Retinoic acid (RA), the end-product of the retinol metabolism pathway, induced in vitro differentiation of ESC into male germ cells. Quantitative real-time PCR (qRT-PCR) was used to detect changes in the expression of the genes involved in the retinol metabolic pathways. From the results of RNA-seq and the database analyses, we concluded that there are 328 genes in 27 lipid metabolic pathways continuously involved in lipid metabolism during the differentiation of ESC into SSC in vivo, including retinol metabolism. Alcohol dehydrogenase 5 (ADH5) and aldehyde dehydrogenase 1 family member A1 (ALDH1A1) are involved in RA synthesis in the cell. ADH5 was specifically expressed in PGC in our experiments and aldehyde dehydrogenase 1 family member A1 (ALDH1A1) persistently increased throughout development. CYP26b1, a member of the cytochrome P450 superfamily, is involved in the degradation of RA. Expression of CYP26b1, in contrast, decreased throughout development. Exogenous RA in the culture medium induced differentiation of ESC to SSC-like cells. The expression patterns of ADH5, ALDH1A1, and CYP26b1 were consistent with RNA-seq results. We conclude that the retinol metabolism pathway plays an important role in the process of chicken male germ cell differentiation." @default.
• W2054981823 created "2016-06-24" @default.
• W2054981823 creator A5003192568 @default.
• W2054981823 creator A5009929030 @default.
• W2054981823 creator A5015511585 @default.
• W2054981823 creator A5027572238 @default.
• W2054981823 creator A5046735223 @default.
• W2054981823 creator A5047070901 @default.
• W2054981823 creator A5052092284 @default.
• W2054981823 creator A5071033926 @default.
• W2054981823 creator A5071798264 @default.
• W2054981823 creator A5077043575 @default.
• W2054981823 date "2015-02-06" @default.
• W2054981823 modified "2023-10-17" @default.
• W2054981823 title "Study on the Regulatory Mechanism of the Lipid Metabolism Pathways during Chicken Male Germ Cell Differentiation Based on RNA-Seq" @default.
• W2054981823 cites W1977135603 @default.
• W2054981823 cites W1979700611 @default.
• W2054981823 cites W1980912680 @default.
• W2054981823 cites W1981509058 @default.
• W2054981823 cites W1984708728 @default.
• W2054981823 cites W1996430474 @default.
• W2054981823 cites W2015138787 @default.
• W2054981823 cites W2027553882 @default.
• W2054981823 cites W2031416354 @default.
• W2054981823 cites W2036654302 @default.
• W2054981823 cites W2039121721 @default.
• W2054981823 cites W2058805572 @default.
• W2054981823 cites W2102806129 @default.
• W2054981823 cites W2104698675 @default.
• W2054981823 cites W2105776281 @default.
• W2054981823 cites W2143023822 @default.
• W2054981823 cites W2154431984 @default.
• W2054981823 cites W2155308712 @default.
• W2054981823 doi "https://doi.org/10.1371/journal.pone.0109469" @default.
• W2054981823 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4320113" @default.
• W2054981823 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25658587" @default.
• W2054981823 hasPublicationYear "2015" @default.
• W2054981823 type Work @default.
• W2054981823 sameAs 2054981823 @default.
• W2054981823 citedByCount "5" @default.
• W2054981823 countsByYear W20549818232017 @default.
• W2054981823 countsByYear W20549818232019 @default.
• W2054981823 countsByYear W20549818232022 @default.
• W2054981823 crossrefType "journal-article" @default.
• W2054981823 hasAuthorship W2054981823A5003192568 @default.
• W2054981823 hasAuthorship W2054981823A5009929030 @default.
• W2054981823 hasAuthorship W2054981823A5015511585 @default.
• W2054981823 hasAuthorship W2054981823A5027572238 @default.
• W2054981823 hasAuthorship W2054981823A5046735223 @default.
• W2054981823 hasAuthorship W2054981823A5047070901 @default.
• W2054981823 hasAuthorship W2054981823A5052092284 @default.
• W2054981823 hasAuthorship W2054981823A5071033926 @default.
• W2054981823 hasAuthorship W2054981823A5071798264 @default.
• W2054981823 hasAuthorship W2054981823A5077043575 @default.
• W2054981823 hasBestOaLocation W20549818231 @default.
• W2054981823 hasConcept C104317684 @default.
• W2054981823 hasConcept C148738053 @default.
• W2054981823 hasConcept C150194340 @default.
• W2054981823 hasConcept C152724338 @default.
• W2054981823 hasConcept C162317418 @default.
• W2054981823 hasConcept C192989942 @default.
• W2054981823 hasConcept C25747580 @default.
• W2054981823 hasConcept C2781121885 @default.
• W2054981823 hasConcept C4733338 @default.
• W2054981823 hasConcept C55493867 @default.
• W2054981823 hasConcept C86803240 @default.
• W2054981823 hasConcept C95444343 @default.
• W2054981823 hasConceptScore W2054981823C104317684 @default.
• W2054981823 hasConceptScore W2054981823C148738053 @default.
• W2054981823 hasConceptScore W2054981823C150194340 @default.
• W2054981823 hasConceptScore W2054981823C152724338 @default.
• W2054981823 hasConceptScore W2054981823C162317418 @default.
• W2054981823 hasConceptScore W2054981823C192989942 @default.
• W2054981823 hasConceptScore W2054981823C25747580 @default.
• W2054981823 hasConceptScore W2054981823C2781121885 @default.
• W2054981823 hasConceptScore W2054981823C4733338 @default.
• W2054981823 hasConceptScore W2054981823C55493867 @default.
• W2054981823 hasConceptScore W2054981823C86803240 @default.
• W2054981823 hasConceptScore W2054981823C95444343 @default.
• W2054981823 hasIssue "2" @default.
• W2054981823 hasLocation W20549818231 @default.
• W2054981823 hasLocation W20549818232 @default.
• W2054981823 hasLocation W20549818233 @default.
• W2054981823 hasLocation W20549818234 @default.
• W2054981823 hasLocation W20549818235 @default.
• W2054981823 hasOpenAccess W2054981823 @default.
• W2054981823 hasPrimaryLocation W20549818231 @default.
• W2054981823 hasRelatedWork W1898393660 @default.
• W2054981823 hasRelatedWork W2315982888 @default.
• W2054981823 hasRelatedWork W2730712845 @default.
• W2054981823 hasRelatedWork W2905155342 @default.
• W2054981823 hasRelatedWork W2998071603 @default.
• W2054981823 hasRelatedWork W3025961873 @default.
• W2054981823 hasRelatedWork W3120266570 @default.
• W2054981823 hasRelatedWork W3123579897 @default.
• W2054981823 hasRelatedWork W4205775939 @default.
• W2054981823 hasRelatedWork W4319780875 @default.
• W2054981823 hasVolume "10" @default.

• next