FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2054996964> ?p ?o ?g. }

• W2054996964 abstract "Abstract Background Micrurus corallinus (coral snake) is a tropical forest snake belonging to the family Elapidae. Its venom shows a high neurotoxicity associated with pre- and post-synaptic toxins, causing diaphragm paralysis, which may result in death. In spite of a relatively small incidence of accidents, serum therapy is crucial for those bitten. However, the adequate production of antiserum is hampered by the difficulty in obtaining sufficient amounts of venom from a small snake with demanding breeding conditions. In order to elucidate the molecular basis of this venom and to uncover possible immunogens for an antiserum, we generated expressed sequences tags (ESTs) from its venom glands and analyzed the transcriptomic profile. In addition, their immunogenicity was tested using DNA immunization. Results A total of 1438 ESTs were generated and grouped into 611 clusters. Toxin transcripts represented 46% of the total ESTs. The two main toxin classes consisted of three-finger toxins (3FTx) (24%) and phospholipases A 2 (PLA 2 s) (15%). However, 8 other classes of toxins were present, including C-type lectins, natriuretic peptide precursors and even high-molecular mass components such as metalloproteases and L-amino acid oxidases. Each class included an assortment of isoforms, some showing evidence of alternative splicing and domain deletions. Five antigenic candidates were selected (four 3FTx and one PLA 2 ) and used for a preliminary study of DNA immunization. The immunological response showed that the sera from the immunized animals were able to recognize the recombinant antigens. Conclusion Besides an improvement in our knowledge of the composition of coral snake venoms, which are very poorly known when compared to Old World elapids, the expression profile suggests abundant and diversified components that may be used in future antiserum formulation. As recombinant production of venom antigens frequently fails due to complex disulfide arrangements, DNA immunization may be a viable alternative. In fact, the selected candidates provided an initial evidence of the feasibility of this approach, which is less costly and not dependent on the availability of the venom." @default.
• W2054996964 created "2016-06-24" @default.
• W2054996964 creator A5056463876 @default.
• W2054996964 creator A5066066519 @default.
• W2054996964 creator A5066365481 @default.
• W2054996964 date "2009-03-16" @default.
• W2054996964 modified "2023-10-13" @default.
• W2054996964 title "Transcriptomic basis for an antiserum against Micrurus corallinus (coral snake) venom" @default.
• W2054996964 cites W1565563859 @default.
• W2054996964 cites W1577981179 @default.
• W2054996964 cites W1591000273 @default.
• W2054996964 cites W1963598677 @default.
• W2054996964 cites W1965877687 @default.
• W2054996964 cites W1973169354 @default.
• W2054996964 cites W1974166565 @default.
• W2054996964 cites W1980428805 @default.
• W2054996964 cites W1980879199 @default.
• W2054996964 cites W1988276853 @default.
• W2054996964 cites W1996416017 @default.
• W2054996964 cites W1996518529 @default.
• W2054996964 cites W1996530412 @default.
• W2054996964 cites W2003443276 @default.
• W2054996964 cites W2011570577 @default.
• W2054996964 cites W2017263271 @default.
• W2054996964 cites W2023888813 @default.
• W2054996964 cites W2025208262 @default.
• W2054996964 cites W2027376251 @default.
• W2054996964 cites W2037457212 @default.
• W2054996964 cites W2043381167 @default.
• W2054996964 cites W2044145349 @default.
• W2054996964 cites W2044506803 @default.
• W2054996964 cites W2050575429 @default.
• W2054996964 cites W2055756635 @default.
• W2054996964 cites W2056877436 @default.
• W2054996964 cites W2066116350 @default.
• W2054996964 cites W2074466720 @default.
• W2054996964 cites W2077340935 @default.
• W2054996964 cites W2081489602 @default.
• W2054996964 cites W2081698356 @default.
• W2054996964 cites W2101994976 @default.
• W2054996964 cites W2106564812 @default.
• W2054996964 cites W2134231543 @default.
• W2054996964 cites W2145325237 @default.
• W2054996964 cites W2159432198 @default.
• W2054996964 cites W2162385837 @default.
• W2054996964 cites W2164025341 @default.
• W2054996964 cites W2166265186 @default.
• W2054996964 cites W2166647530 @default.
• W2054996964 cites W2416844230 @default.
• W2054996964 doi "https://doi.org/10.1186/1471-2164-10-112" @default.
• W2054996964 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2662881" @default.
• W2054996964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19291316" @default.
• W2054996964 hasPublicationYear "2009" @default.
• W2054996964 type Work @default.
• W2054996964 sameAs 2054996964 @default.
• W2054996964 citedByCount "51" @default.
• W2054996964 countsByYear W20549969642012 @default.
• W2054996964 countsByYear W20549969642013 @default.
• W2054996964 countsByYear W20549969642014 @default.
• W2054996964 countsByYear W20549969642015 @default.
• W2054996964 countsByYear W20549969642016 @default.
• W2054996964 countsByYear W20549969642017 @default.
• W2054996964 countsByYear W20549969642018 @default.
• W2054996964 countsByYear W20549969642019 @default.
• W2054996964 countsByYear W20549969642021 @default.
• W2054996964 countsByYear W20549969642022 @default.
• W2054996964 countsByYear W20549969642023 @default.
• W2054996964 crossrefType "journal-article" @default.
• W2054996964 hasAuthorship W2054996964A5056463876 @default.
• W2054996964 hasAuthorship W2054996964A5066066519 @default.
• W2054996964 hasAuthorship W2054996964A5066365481 @default.
• W2054996964 hasBestOaLocation W20549969641 @default.
• W2054996964 hasConcept C117287731 @default.
• W2054996964 hasConcept C145600735 @default.
• W2054996964 hasConcept C147483822 @default.
• W2054996964 hasConcept C2776725428 @default.
• W2054996964 hasConcept C2777009914 @default.
• W2054996964 hasConcept C2779448229 @default.
• W2054996964 hasConcept C2780868878 @default.
• W2054996964 hasConcept C54355233 @default.
• W2054996964 hasConcept C55493867 @default.
• W2054996964 hasConcept C86803240 @default.
• W2054996964 hasConcept C91523082 @default.
• W2054996964 hasConceptScore W2054996964C117287731 @default.
• W2054996964 hasConceptScore W2054996964C145600735 @default.
• W2054996964 hasConceptScore W2054996964C147483822 @default.
• W2054996964 hasConceptScore W2054996964C2776725428 @default.
• W2054996964 hasConceptScore W2054996964C2777009914 @default.
• W2054996964 hasConceptScore W2054996964C2779448229 @default.
• W2054996964 hasConceptScore W2054996964C2780868878 @default.
• W2054996964 hasConceptScore W2054996964C54355233 @default.
• W2054996964 hasConceptScore W2054996964C55493867 @default.
• W2054996964 hasConceptScore W2054996964C86803240 @default.
• W2054996964 hasConceptScore W2054996964C91523082 @default.
• W2054996964 hasIssue "1" @default.
• W2054996964 hasLocation W20549969641 @default.
• W2054996964 hasLocation W20549969642 @default.
• W2054996964 hasLocation W20549969643 @default.
• W2054996964 hasLocation W20549969644 @default.
• W2054996964 hasOpenAccess W2054996964 @default.

• next