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• W2055029944 abstract "Abstract Inoculation into chicken embryos of chick erythrocytes infected with Plasmodium gallinaceum (the infected erythrocytes having been obtained from chicks inoculated with sporozoites) produced parasitemias of such low magnitude that it was impossible to continue embryo passages of this malaria. Inoculation of massive doses of parasites (20 × 10 6 ) produced levels of infections in recipient embryos sufficient to maintain continued passages. Marked morphological changes in parasites were noted as well as loss of virulence. Well over 100 embryo passages were required to restore the parasite to its usual virulence and morphology. When chicks were given subcutaneous injections of this embryo-adapted strain of malaria at an optimum dosage of 25 × 10 4 parasites/g body weight, the resultant parasitemia was too low for accurate counting, but all animals so treated were completely refractory to challenge with blood parasites. Although protection was complete in chicks vaccinated with parasites from the 13th passage in embryos, vaccinating dosages produced levels of parasitemia too high to be judged adequate for a vaccine. After 24 embryo passages attenuation of the parasite and immunity to challenge on the part of the host were complete." @default.
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• W2055029944 date "1977-10-01" @default.
• W2055029944 modified "2023-09-24" @default.
• W2055029944 title "Plasmodium gallinaceum: Vaccination in chickens" @default.
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• W2055029944 doi "https://doi.org/10.1016/0014-4894(77)90027-3" @default.
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