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- W2055085914 abstract "We have synthesized the deoxyadenosine derivative tethering a phenyl group (X), which mimics the Watson-Crick A/T base pair. The RNA/DNA hybrid duplexes containing X in the middle of the DNA sequence showed a similar thermal stability regardless of the ribonucleotide species (A, G, C, or U) opposite to X, probably because of the phenyl group stacking inside of the duplex accompanied by the opposite ribonucleotide base flipped in an extrahelical position. The RNA strand hybridized with the DNA strand bearing X was cleaved on the 3'-side of the ribonucleotide opposite to X in the presence of MgCl2, and the RNA sequence to be cleaved was not restricted. The site-specific RNA hydrolysis suggests that the DNA strand bearing X has the advantage of the site-selective base flipping in the target sequence and the development of a universal deoxyribozyme to exclusively cleave a target RNA sequence." @default.
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- W2055085914 date "2004-12-23" @default.
- W2055085914 modified "2023-09-27" @default.
- W2055085914 title "Site-Selective RNA Cleavage by DNA Bearing a Base Pair-Mimic Nucleoside" @default.
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- W2055085914 doi "https://doi.org/10.1021/ja045445s" @default.
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