Matches in SemOpenAlex for { <https://semopenalex.org/work/W2055120717> ?p ?o ?g. }
- W2055120717 endingPage "195" @default.
- W2055120717 startingPage "176" @default.
- W2055120717 abstract "Abstract Investigating stages of maturation of cellular adhesions to the extracellular matrix from the initial binding events to the formation of small focal complexes has been challenging because of the difficulty in fabricating the necessary nanopatterned substrates with controlled biochemical functionality. We present the fabrication and characterization of surfaces presenting fibronectin nanopatterns of controlled size and pitch that provide well‐defined cellular adhesion sites against a nonadhesive polyethylene glycol background. The nanopatterned surfaces allow us to control the number of fibronectin proteins within each adhesion site from 9 to 250, thereby limiting the number of integrins involved in each cell–substrate adhesion. We demonstrate the presence of fibronectin on the nanoislands, while no protein was observed on the passivated background. We show that the cell adheres to the nanopatterns with adhesions that are much smaller and more evenly distributed than on a glass control. The nanopattern influences cellular proliferation only at longer times, but influences spreading at both early and later times, indicating adhesion size and adhesion density play a role in controlling cell adhesion and signaling. However, the overall density of fibronectin on all patterns is far lower than on homogeneously coated control surfaces, showing that the local density of adhesion ligands, not the average density, is the important parameter for cell proliferation and spreading. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 87A: 176–195, 2008" @default.
- W2055120717 created "2016-06-24" @default.
- W2055120717 creator A5029729155 @default.
- W2055120717 creator A5090471164 @default.
- W2055120717 date "2007-12-17" @default.
- W2055120717 modified "2023-10-10" @default.
- W2055120717 title "Nanopatterning of fibronectin and the influence of integrin clustering on endothelial cell spreading and proliferation" @default.
- W2055120717 cites W1499411251 @default.
- W2055120717 cites W1514050185 @default.
- W2055120717 cites W1555914875 @default.
- W2055120717 cites W1590619267 @default.
- W2055120717 cites W1594669421 @default.
- W2055120717 cites W1748298985 @default.
- W2055120717 cites W1844137891 @default.
- W2055120717 cites W1927705072 @default.
- W2055120717 cites W1929023701 @default.
- W2055120717 cites W1963892111 @default.
- W2055120717 cites W1965933207 @default.
- W2055120717 cites W1966296130 @default.
- W2055120717 cites W1967060076 @default.
- W2055120717 cites W1967195782 @default.
- W2055120717 cites W1971106165 @default.
- W2055120717 cites W1972341508 @default.
- W2055120717 cites W1983426119 @default.
- W2055120717 cites W1983842862 @default.
- W2055120717 cites W1986681332 @default.
- W2055120717 cites W1986779411 @default.
- W2055120717 cites W1989124188 @default.
- W2055120717 cites W1990038274 @default.
- W2055120717 cites W1992563783 @default.
- W2055120717 cites W1993799862 @default.
- W2055120717 cites W1996945417 @default.
- W2055120717 cites W2000763360 @default.
- W2055120717 cites W2003321757 @default.
- W2055120717 cites W2005228179 @default.
- W2055120717 cites W2011848496 @default.
- W2055120717 cites W2013272129 @default.
- W2055120717 cites W2013744373 @default.
- W2055120717 cites W2015498249 @default.
- W2055120717 cites W2017330811 @default.
- W2055120717 cites W2019384864 @default.
- W2055120717 cites W2021826257 @default.
- W2055120717 cites W2022339318 @default.
- W2055120717 cites W2022511137 @default.
- W2055120717 cites W2024885580 @default.
- W2055120717 cites W2026400584 @default.
- W2055120717 cites W2027466368 @default.
- W2055120717 cites W2028936118 @default.
- W2055120717 cites W2029231027 @default.
- W2055120717 cites W2030550786 @default.
- W2055120717 cites W2032275376 @default.
- W2055120717 cites W2032392940 @default.
- W2055120717 cites W2033273930 @default.
- W2055120717 cites W2033896550 @default.
- W2055120717 cites W2035236528 @default.
- W2055120717 cites W2036220159 @default.
- W2055120717 cites W2036794574 @default.
- W2055120717 cites W2039347039 @default.
- W2055120717 cites W2042076247 @default.
- W2055120717 cites W2042210454 @default.
- W2055120717 cites W2042301280 @default.
- W2055120717 cites W2042477060 @default.
- W2055120717 cites W2045367131 @default.
- W2055120717 cites W2045383620 @default.
- W2055120717 cites W2053001031 @default.
- W2055120717 cites W2053884368 @default.
- W2055120717 cites W2054635074 @default.
- W2055120717 cites W2057614629 @default.
- W2055120717 cites W2057936850 @default.
- W2055120717 cites W2058096164 @default.
- W2055120717 cites W2060548956 @default.
- W2055120717 cites W2061158555 @default.
- W2055120717 cites W2061487542 @default.
- W2055120717 cites W2061833861 @default.
- W2055120717 cites W2064186538 @default.
- W2055120717 cites W2064768989 @default.
- W2055120717 cites W2065317828 @default.
- W2055120717 cites W2069284563 @default.
- W2055120717 cites W2069601286 @default.
- W2055120717 cites W2072679798 @default.
- W2055120717 cites W2073561679 @default.
- W2055120717 cites W2074352273 @default.
- W2055120717 cites W2076580477 @default.
- W2055120717 cites W2076590551 @default.
- W2055120717 cites W2078689444 @default.
- W2055120717 cites W2082695261 @default.
- W2055120717 cites W2089190026 @default.
- W2055120717 cites W2091295830 @default.
- W2055120717 cites W2094370158 @default.
- W2055120717 cites W2098573045 @default.
- W2055120717 cites W2099052265 @default.
- W2055120717 cites W2104527243 @default.
- W2055120717 cites W2105181228 @default.
- W2055120717 cites W2107802998 @default.
- W2055120717 cites W2110981204 @default.
- W2055120717 cites W2111811045 @default.
- W2055120717 cites W2112820767 @default.
- W2055120717 cites W2113641476 @default.