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• W2055141898 abstract "Many of the deaths caused by tuberculosis (TB) in the world are due to wrong or late diagnosis. The World Health Organization (WHO) calls for better and cheaper TB tests method for this reason. In this paper, a new multi-channel series piezoelectric quartz crystal (MSPQC) sensor system was developed for rapid growth and detection of Mycobacterium tuberculosis. It is an automatic continuous monitoring system. The system was used to detect TB based on the volatile metabolic products NH3 and CO2 during the growth of M. tuberculosis. The metabolic products, diffusing from the medium into the KOH absorbing solution, resulted in the conductance change of the absorbing solution detected by the MSPQC sensitively. The frequency shift versus time response curves were recorded by self-developed software. Frequency detection time (FDT) corresponding to −100 Hz in frequency shift value was used as a parameter to quantitatively determine M. tuberculosis H37Ra (an avirulent strain). H37Ra and 40 strains clinic positive samples were detected by the proposed system successfully. As for H37Ra, the FDT had a linear relationship with the logarithm of its initial concentration in the range of 102–107 colony forming units (cfu) ml−1 (R = −0.998) and the detection limit was low to 10 cfu ml−1. 4% NaOH solution that can kill contaminating microorganisms and make M. tuberculosis alive was used as pretreatment reagent to provide selectivity to this method. Comparative tests were also carried out by using BACTEC™ MGIT™ 960 and conventional Lowenstein–Jensen (L–J) slants. The results showed that the proposed system was quicker than BACTEC™ MGIT™ 960 and it is also cheaper and will be widely used in TB tests in the world." @default.
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• W2055141898 date "2008-11-01" @default.
• W2055141898 modified "2023-10-18" @default.
• W2055141898 title "A new MSPQC for rapid growth and detection of Mycobacterium tuberculosis" @default.
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• W2055141898 doi "https://doi.org/10.1016/j.bios.2008.04.018" @default.
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