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- W2055142458 abstract "Abstract The relative sensitivity of phase‐cycled and gradient‐selected HMBC spectra is assessed. As expected, the gradient‐selected sequence is clearly superior to the phase‐cycled sequence for concentrated solutions where t 1 ridges due to incomplete suppression of 1 H magnetization bonded to 13 C or heteroatoms are the main sources of noise in the phase‐cycled spectrum but are strongly suppressed in the gradient‐selected spectrum. However, the intensity of t 1 ridges appears to be directly proportional to signal strength. Consequently, for dilute solutions, t 1 ridges often provide only a minor contribution to total noise levels in phase‐cycled HMBC spectra. In this case, provided that one acquires and processes phase‐cycled HMBC spectra in the recommended mode (phase‐sensitive acquisition and mixed‐mode processing), a phase‐cycled HMBC spectrum can show about twice the signal‐to‐noise ratio of an absolute value gradient‐selected HMBC obtained in the same time. More extensive linear prediction is also possible with the phase‐cycled sequence. There are similar advantages to phase‐cycled HSQC spectra over gradient‐selected HSQC spectra. Copyright © 2001 John Wiley & Sons, Ltd." @default.
- W2055142458 created "2016-06-24" @default.
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- W2055142458 date "2001-08-10" @default.
- W2055142458 modified "2023-10-02" @default.
- W2055142458 title "Gradient-selected versus phase-cycled HMBC and HSQC: pros and cons" @default.
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- W2055142458 doi "https://doi.org/10.1002/mrc.884" @default.
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