Matches in SemOpenAlex for { <https://semopenalex.org/work/W2055143571> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2055143571 endingPage "92" @default.
- W2055143571 startingPage "81" @default.
- W2055143571 abstract "A selective, sensitive and fully automated column-switching LC system using direct injection of human plasma followed by mass spectrometry (MS) detection was developed and validated to determine the concentrations of 4-demethoxy-3'-deamino-3'-aziridinyl-4'-methylsulphonyldaunorubicin++ + (PNU-159548) and its 13-hydroxy metabolite (PNU-169884). A 50-microl human plasma sample was directly introduced into a C4-alkyl-diol silica clean-up column separating analytes from proteins and polar endogenous compounds using water and methanol as the mobile phase. The fraction containing PNU-159548 and its metabolite was back-flushed and transferred to the analytical column. The compounds were separated using a Zorbax SB C8 column (150x4.6 mm, 5 microm) under gradient conditions with the mobile phase containing acetonitrile and 2 mM ammonium formate, pH 3.5. MS detection was by atmospheric pressure ionisation with multiple reaction monitoring in positive ion mode. Linearity was demonstrated over the calibration range of 0.051-10.291 ng/ml for PNU-159548 and 0.104-10.434 ng/ml for PNU-169884. The assay was validated with respect to accuracy, precision and analyte stability. On the basis of the validation data, the developed analytical method was found to be suitable for use in Phase I clinical studies." @default.
- W2055143571 created "2016-06-24" @default.
- W2055143571 creator A5012312631 @default.
- W2055143571 creator A5052340231 @default.
- W2055143571 creator A5053567544 @default.
- W2055143571 creator A5072410573 @default.
- W2055143571 creator A5082791842 @default.
- W2055143571 date "1999-08-01" @default.
- W2055143571 modified "2023-10-17" @default.
- W2055143571 title "Determination of 4-demethoxy-3′-deamino-3′-aziridinyl-4′-methylsulphonyldaunorubicin and its 13-hydroxy metabolite by direct injection of human plasma into a column-switching liquid chromatography system with mass spectrometric detection" @default.
- W2055143571 cites W1986585517 @default.
- W2055143571 cites W2039993350 @default.
- W2055143571 cites W2056427715 @default.
- W2055143571 doi "https://doi.org/10.1016/s0021-9673(99)00748-7" @default.
- W2055143571 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10497930" @default.
- W2055143571 hasPublicationYear "1999" @default.
- W2055143571 type Work @default.
- W2055143571 sameAs 2055143571 @default.
- W2055143571 citedByCount "11" @default.
- W2055143571 crossrefType "journal-article" @default.
- W2055143571 hasAuthorship W2055143571A5012312631 @default.
- W2055143571 hasAuthorship W2055143571A5052340231 @default.
- W2055143571 hasAuthorship W2055143571A5053567544 @default.
- W2055143571 hasAuthorship W2055143571A5072410573 @default.
- W2055143571 hasAuthorship W2055143571A5082791842 @default.
- W2055143571 hasConcept C10390740 @default.
- W2055143571 hasConcept C113196181 @default.
- W2055143571 hasConcept C119128265 @default.
- W2055143571 hasConcept C162356407 @default.
- W2055143571 hasConcept C179998833 @default.
- W2055143571 hasConcept C185592680 @default.
- W2055143571 hasConcept C2775986502 @default.
- W2055143571 hasConcept C2777463227 @default.
- W2055143571 hasConcept C2777477808 @default.
- W2055143571 hasConcept C2778576202 @default.
- W2055143571 hasConcept C31827203 @default.
- W2055143571 hasConcept C43617362 @default.
- W2055143571 hasConcept C55493867 @default.
- W2055143571 hasConcept C71043704 @default.
- W2055143571 hasConcept C95986675 @default.
- W2055143571 hasConceptScore W2055143571C10390740 @default.
- W2055143571 hasConceptScore W2055143571C113196181 @default.
- W2055143571 hasConceptScore W2055143571C119128265 @default.
- W2055143571 hasConceptScore W2055143571C162356407 @default.
- W2055143571 hasConceptScore W2055143571C179998833 @default.
- W2055143571 hasConceptScore W2055143571C185592680 @default.
- W2055143571 hasConceptScore W2055143571C2775986502 @default.
- W2055143571 hasConceptScore W2055143571C2777463227 @default.
- W2055143571 hasConceptScore W2055143571C2777477808 @default.
- W2055143571 hasConceptScore W2055143571C2778576202 @default.
- W2055143571 hasConceptScore W2055143571C31827203 @default.
- W2055143571 hasConceptScore W2055143571C43617362 @default.
- W2055143571 hasConceptScore W2055143571C55493867 @default.
- W2055143571 hasConceptScore W2055143571C71043704 @default.
- W2055143571 hasConceptScore W2055143571C95986675 @default.
- W2055143571 hasIssue "1-2" @default.
- W2055143571 hasLocation W20551435711 @default.
- W2055143571 hasLocation W20551435712 @default.
- W2055143571 hasOpenAccess W2055143571 @default.
- W2055143571 hasPrimaryLocation W20551435711 @default.
- W2055143571 hasRelatedWork W1964888264 @default.
- W2055143571 hasRelatedWork W1973892552 @default.
- W2055143571 hasRelatedWork W1994074014 @default.
- W2055143571 hasRelatedWork W2022369336 @default.
- W2055143571 hasRelatedWork W2039494622 @default.
- W2055143571 hasRelatedWork W2049606890 @default.
- W2055143571 hasRelatedWork W2078342120 @default.
- W2055143571 hasRelatedWork W2082182980 @default.
- W2055143571 hasRelatedWork W2553693764 @default.
- W2055143571 hasRelatedWork W29133270 @default.
- W2055143571 hasVolume "854" @default.
- W2055143571 isParatext "false" @default.
- W2055143571 isRetracted "false" @default.
- W2055143571 magId "2055143571" @default.
- W2055143571 workType "article" @default.