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- W2055184252 abstract "A new method specific for the determination of subpicomole quantities of tryptophan has been developed by elaboration of the Pictet-Spengler reaction. It permitted reproducible quantitation of tryptophan in less than 1 μl of plasma ultrafiltrate or 1 mg of brain tissue. Samples deproteinized by trichloroacetic acid were boiled for 15 min with formaldehyde and potassium ferricyanide at controlled acidity, where tryptophan was converted to a single new product identified as 9-hydroxymethyl-β-carboline. It was quantitated by either direct spectrofluorometry or a reversed-phase HPLC system developed for β-carbolines. Under our conditions, peptides containing N-terminal tryptophan such as Trp-Leu and delta sleep-inducing peptide gave N-(9-hydroxymethyl-β-carboline-3-carbonyl) peptides which retained all amino acid residues except tryptophan." @default.
- W2055184252 created "2016-06-24" @default.
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- W2055184252 date "1983-07-01" @default.
- W2055184252 modified "2023-09-27" @default.
- W2055184252 title "Picomole analyses of tryptophan by derivatization to 9-hydroxymethyl-β-carboline" @default.
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- W2055184252 doi "https://doi.org/10.1016/0003-2697(83)90036-2" @default.
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