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- W2055211056 abstract "Vacuoles were examined in cells and protoplasts of a fast growing tomato (Lycopersicon esculentum cv. Lukullus) cell suspension culture by light and fluorescence microscopy. In protoplasts prepared from cells of the exponential growth phase a great number of small vacuoles occurred. The number of vacuoles decreased during the cell cycle until a single large vacuole was observed. Vacuoles were released from protoplasts prepared from cells of the late exponential growth phase (3.5 days) by the action of DEAE-dextran or by shaking in an alkaline buffer containing EDTA and purified by centrifugation in discontinuous sorbitol/sucrose/Fi-coll-gradients. Only slight or no contamination of the vacuolar fraction by cytosol, mitochondria, and microbodies was shown by use of marker enzymes. Vacuoles contained approximately 2.5% each of DNA and RNA. α-mannosidase was located exclusively in the vacuole and served as a convenient marker. Acid proteinase and β-N-acetylglucosaminidase were highly enriched in isolated vacuoles whereas acid phosphatase activity was low. During repeated centrifugation of isolated vacuoles about 35% were destroyed at each step. Vacuoles could be kept at 4°C for 6 hours and at 30°C for 1 hour without changing their centrifugation properties if the buffers contained 1 mM each of MgCl2, CaCl2, and 0.5% (w/v) human serum albumine." @default.
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- W2055211056 date "1984-01-01" @default.
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- W2055211056 title "Vacuoles From Cell Suspension Cultures of Tomato (Lycopersicon esculentum) — Isolation and Characterization" @default.
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- W2055211056 doi "https://doi.org/10.1016/s0044-328x(84)80050-1" @default.
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