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• W2055223854 endingPage "371" @default.
• W2055223854 startingPage "361" @default.
• W2055223854 abstract "Spectroscopic studies were performed on the fluorescent sterol probes ergosta-5,7,9(11),22-tetraen-3β-ol (dehydroergosterol) and cholesta-5,7,9(11)-trien-3β-ol (cholestatrienol). In most isotropic solvents, these molecules exhibited a single lifetime near 300 ps. Fluorescence lifetimes in 2-propanol were independent of emission wavelength and independent of excitation wavelength. Excited state behavior of these probes appears relatively simple. In isotropic solvents, dehydroergosterol fluorescence emission underwent at most a small Stokes shift as solvent polarity was modified. Time-resolved anisotropy decays indicated that dehydroergosterol decay was monoexponential, with rotational correlation times dependent on solvent viscosity. When incorporated into l-α-dimyristoylphosphatidylcholine liposomes at a concentration of 0.9 mol%, dehydroergosterol fluorescence lifetime decreased at the phase transition of this phospholipid indicating that the sterol probe was detecting physical changes of the bulk phospholipids. Furthermore, total fluorescence decays and anisotropy decays were sensitive to the environment of the sterol. Dehydroergosterol and cholestatrienol are thus useful probes for monitoring sterol behavior in biological systems." @default.
• W2055223854 created "2016-06-24" @default.
• W2055223854 creator A5042027840 @default.
• W2055223854 creator A5049880042 @default.
• W2055223854 creator A5081854280 @default.
• W2055223854 date "1986-11-01" @default.
• W2055223854 modified "2023-09-27" @default.
• W2055223854 title "Physical properties of the fluorescent sterol probe dehydroergosterol" @default.
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• W2055223854 doi "https://doi.org/10.1016/0005-2736(86)90239-7" @default.
• W2055223854 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3778897" @default.
• W2055223854 hasPublicationYear "1986" @default.
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