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- W2055225023 abstract "A square-wave cathodic stripping voltammetry method for alanine determination as its phenylthiohydantoin (PTH-alanine) derivative is developed. To this end, all the chemical and instrumental variables affecting the determination of PTH-alanine are optimized. From studies of the mechanisms governing the electrochemical response of PTH-alanine, it was concluded that it is an electrochemically irreversible system with a diffusive-adsorptive reduction phenomenon. Under optimal conditions, the variation of analytical signal (I(p)) with PTH-alanine concentration is linear in the 2.4x10(-8)-4.8x10(-7) M range, with a LOD of 1.2x10(-8) M and a LOQ of 4.2x10(-8) M, a RSD (%) less than 11%, and a E(r) (%) less than 10%. The optimized method was applied to the determination of PTH-alanine obtained from a synthetic protein after Edman reaction and the results were corroborated by high-performance liquid chromatography with UV detection." @default.
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- W2055225023 date "2008-08-01" @default.
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- W2055225023 title "Electrochemical analysis of the alanine phenylthiohydantoin derivative by cathodic stripping voltammetry" @default.
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- W2055225023 doi "https://doi.org/10.1016/j.ab.2008.04.046" @default.
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