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• W2055261625 abstract "Gene therapy with plasmid DNA is emerging as a promising strategy for the treatment of many diseases. One of the major obstacles to such therapy is the poor transfection efficiency of DNA in vivo.In this report, we employed a very low power, near-infrared femtosecond laser technique to enhance the transfection efficiency of intradermally and intratumorally administered DNA plasmid.We found that femtosecond laser treatment can significantly enhance the delivery of DNA into the skin and into established tumors in mice. In addition, we found that both laser power density as well as duration of laser treatment are critical parameters for augmenting DNA transfection efficiency. The femtosecond laser technique employs a relatively unfocused laser beam that maximizes the transfected area, minimizes damage to tissue and simplifies its implementation.This femtosecond new laser technology represents a safe and innovative technology for enhancing DNA gene transfer in vivo." @default.
• W2055261625 created "2016-06-24" @default.
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• W2055261625 date "2009-04-01" @default.
• W2055261625 modified "2023-10-13" @default.
• W2055261625 title "Femtosecond laser treatment enhances DNA transfection efficiency in vivo" @default.
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• W2055261625 doi "https://doi.org/10.1186/1423-0127-16-36" @default.
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