Matches in SemOpenAlex for { <https://semopenalex.org/work/W2055269320> ?p ?o ?g. }
- W2055269320 endingPage "907" @default.
- W2055269320 startingPage "900" @default.
- W2055269320 abstract "The 70-kilodalton (kDa) heat-shock proteins (Hsp70s) are ubiquitous molecular chaperones essential for cellular protein folding and proteostasis. Each Hsp70 has two functional domains: a nucleotide-binding domain (NBD), which binds and hydrolyzes ATP, and a substrate-binding domain (SBD), which binds extended polypeptides. NBD and SBD interact little when in the presence of ADP; however, ATP binding allosterically couples the polypeptide- and ATP-binding sites. ATP binding promotes polypeptide release; polypeptide rebinding stimulates ATP hydrolysis. This allosteric coupling is poorly understood. Here we present the crystal structure of an intact ATP-bound Hsp70 from Escherichia coli at 1.96-Å resolution. The ATP-bound NBD adopts a unique conformation, forming extensive interfaces with an SBD that has changed radically, having its α-helical lid displaced and the polypeptide-binding channel of its β-subdomain restructured. These conformational changes, together with our biochemical assays, provide a structural explanation for allosteric coupling in Hsp70 activity." @default.
- W2055269320 created "2016-06-24" @default.
- W2055269320 creator A5003413476 @default.
- W2055269320 creator A5004609678 @default.
- W2055269320 creator A5004763755 @default.
- W2055269320 creator A5009049832 @default.
- W2055269320 creator A5012943964 @default.
- W2055269320 creator A5019693747 @default.
- W2055269320 creator A5024256704 @default.
- W2055269320 creator A5030659071 @default.
- W2055269320 creator A5031425034 @default.
- W2055269320 creator A5040113983 @default.
- W2055269320 creator A5055347404 @default.
- W2055269320 creator A5066887020 @default.
- W2055269320 date "2013-05-26" @default.
- W2055269320 modified "2023-10-01" @default.
- W2055269320 title "Allosteric opening of the polypeptide-binding site when an Hsp70 binds ATP" @default.
- W2055269320 cites W1492302030 @default.
- W2055269320 cites W1527487488 @default.
- W2055269320 cites W1539796472 @default.
- W2055269320 cites W1567164927 @default.
- W2055269320 cites W1789882194 @default.
- W2055269320 cites W1963770237 @default.
- W2055269320 cites W1964995688 @default.
- W2055269320 cites W1967960279 @default.
- W2055269320 cites W1969264987 @default.
- W2055269320 cites W1969650644 @default.
- W2055269320 cites W1969672293 @default.
- W2055269320 cites W1971108880 @default.
- W2055269320 cites W1972770744 @default.
- W2055269320 cites W1974036723 @default.
- W2055269320 cites W1976476593 @default.
- W2055269320 cites W1982991372 @default.
- W2055269320 cites W1983544779 @default.
- W2055269320 cites W1983732912 @default.
- W2055269320 cites W1986191025 @default.
- W2055269320 cites W1989189288 @default.
- W2055269320 cites W1991970381 @default.
- W2055269320 cites W1996623614 @default.
- W2055269320 cites W1999268424 @default.
- W2055269320 cites W2007959056 @default.
- W2055269320 cites W2014294043 @default.
- W2055269320 cites W2028705478 @default.
- W2055269320 cites W2029265132 @default.
- W2055269320 cites W2034943952 @default.
- W2055269320 cites W2039975390 @default.
- W2055269320 cites W2042232940 @default.
- W2055269320 cites W2042838776 @default.
- W2055269320 cites W2045548261 @default.
- W2055269320 cites W2045872211 @default.
- W2055269320 cites W2046629597 @default.
- W2055269320 cites W2048350644 @default.
- W2055269320 cites W2051592058 @default.
- W2055269320 cites W2053544172 @default.
- W2055269320 cites W2063502932 @default.
- W2055269320 cites W2064292796 @default.
- W2055269320 cites W2064524157 @default.
- W2055269320 cites W2064676619 @default.
- W2055269320 cites W2064878094 @default.
- W2055269320 cites W2073580695 @default.
- W2055269320 cites W2074986801 @default.
- W2055269320 cites W2076159058 @default.
- W2055269320 cites W2076410859 @default.
- W2055269320 cites W2076945013 @default.
- W2055269320 cites W2081231020 @default.
- W2055269320 cites W2082846873 @default.
- W2055269320 cites W2085703132 @default.
- W2055269320 cites W2089457735 @default.
- W2055269320 cites W2092198909 @default.
- W2055269320 cites W2107334607 @default.
- W2055269320 cites W2109052254 @default.
- W2055269320 cites W2109310563 @default.
- W2055269320 cites W2122994276 @default.
- W2055269320 cites W2123768693 @default.
- W2055269320 cites W2144081223 @default.
- W2055269320 cites W2145897869 @default.
- W2055269320 cites W2154714625 @default.
- W2055269320 cites W2157771392 @default.
- W2055269320 cites W2160451368 @default.
- W2055269320 cites W2168009822 @default.
- W2055269320 cites W4248872320 @default.
- W2055269320 doi "https://doi.org/10.1038/nsmb.2583" @default.
- W2055269320 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3772632" @default.
- W2055269320 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23708608" @default.
- W2055269320 hasPublicationYear "2013" @default.
- W2055269320 type Work @default.
- W2055269320 sameAs 2055269320 @default.
- W2055269320 citedByCount "205" @default.
- W2055269320 countsByYear W20552693202013 @default.
- W2055269320 countsByYear W20552693202014 @default.
- W2055269320 countsByYear W20552693202015 @default.
- W2055269320 countsByYear W20552693202016 @default.
- W2055269320 countsByYear W20552693202017 @default.
- W2055269320 countsByYear W20552693202018 @default.
- W2055269320 countsByYear W20552693202019 @default.
- W2055269320 countsByYear W20552693202020 @default.
- W2055269320 countsByYear W20552693202021 @default.
- W2055269320 countsByYear W20552693202022 @default.