FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2055362838> ?p ?o ?g. }

• W2055362838 endingPage "268" @default.
• W2055362838 startingPage "260" @default.
• W2055362838 abstract "Understanding the mechanisms regulating expression of retinal ganglion cell (RGC) specific and axon-guidance genes during development and in retinal stem cells will be critical for successful optic nerve regeneration. Müller glia have some characteristics of retinal stem cells but in mammals have demonstrated limited potential to differentiate into RGCs. Chromatin remodeling through histone deacetylation and DNA methylation are a potential mechanism for silencing genes necessary for neuronal differentiation of glial cells. We investigated DNA methylation as a mechanism for regulating expression of mouse EphA5, one member of a large family of ephrin receptor genes that regulate patterning of the topographic connections of RGCs during visual system development. We analyzed spatial and age-related patterns of EphA5 promoter methylation by bisulfite sequencing and mRNA expression by quantitative RT-PCR in the mouse retina. The CpG island in the EphA5 promoter was hypomethylated in the retina and showed no change in overall methylation with age, despite a decline in EphA5 mRNA expression levels in the adult retina. In the nasal retina of post-natal day 0 mice, there was a modest, but statistically significant increase in methylation. Increased methylation corresponded with lower levels of receptor mRNA expression in the nasal retina. We cloned the EphA5 promoter and found that site-specific differences in methylation could preferentially activate or repress promoter activity in transient transfections of rat retinal progenitor cells (R28) using luciferase assays. In sphere cultures generated by EGF/FGF2 stimulation of conditionally immortalized mouse Müller glia (ImM10), EphA5 promoter was hypermethylated and EphA5 mRNA was not detected. Demethylation using 5-azadeoxycytidine (AzadC) resulted in a significant decrease of methylation of the EphA5 promoter and re-expression of the EphA5 mRNA. The inverse relationship between EphA5 promoter methylation and mRNA expression is consistent with a role for DNA methylation in modulating the spatial patterns of EphA5 gene expression in the retina and in silencing EphA5 expression in ImM10 cells. The robust up-regulation of EphA5 in ImM10 cells following demethylation suggests that modulation of chromatin structure may be a useful approach for promoting expression of silenced developmental genes and increasing the neurogenic potential of Müller glia." @default.
• W2055362838 created "2016-06-24" @default.
• W2055362838 creator A5030506046 @default.
• W2055362838 creator A5043109858 @default.
• W2055362838 creator A5083470381 @default.
• W2055362838 date "2011-01-01" @default.
• W2055362838 modified "2023-10-17" @default.
• W2055362838 title "A role for DNA methylation in regulation of EphA5 receptor expression in the mouse retina" @default.
• W2055362838 cites W1527110415 @default.
• W2055362838 cites W1536643155 @default.
• W2055362838 cites W1556791620 @default.
• W2055362838 cites W1893444436 @default.
• W2055362838 cites W1895565798 @default.
• W2055362838 cites W1965139980 @default.
• W2055362838 cites W1966339894 @default.
• W2055362838 cites W1978148843 @default.
• W2055362838 cites W1982237034 @default.
• W2055362838 cites W1983479163 @default.
• W2055362838 cites W1983786929 @default.
• W2055362838 cites W1986224715 @default.
• W2055362838 cites W1986383569 @default.
• W2055362838 cites W2002334405 @default.
• W2055362838 cites W2002409269 @default.
• W2055362838 cites W2004181770 @default.
• W2055362838 cites W2005399401 @default.
• W2055362838 cites W2005423313 @default.
• W2055362838 cites W2005458378 @default.
• W2055362838 cites W2013633022 @default.
• W2055362838 cites W2015347860 @default.
• W2055362838 cites W2015722340 @default.
• W2055362838 cites W2015966587 @default.
• W2055362838 cites W2016508121 @default.
• W2055362838 cites W2016550440 @default.
• W2055362838 cites W2025722914 @default.
• W2055362838 cites W2030498911 @default.
• W2055362838 cites W2032692272 @default.
• W2055362838 cites W2032911272 @default.
• W2055362838 cites W2033585891 @default.
• W2055362838 cites W2037577105 @default.
• W2055362838 cites W2037846776 @default.
• W2055362838 cites W2038531539 @default.
• W2055362838 cites W2040474954 @default.
• W2055362838 cites W2055522965 @default.
• W2055362838 cites W2055558351 @default.
• W2055362838 cites W2056418457 @default.
• W2055362838 cites W2060648351 @default.
• W2055362838 cites W2061115389 @default.
• W2055362838 cites W2063504478 @default.
• W2055362838 cites W2065484964 @default.
• W2055362838 cites W2066693652 @default.
• W2055362838 cites W2072826787 @default.
• W2055362838 cites W2078686328 @default.
• W2055362838 cites W2079027682 @default.
• W2055362838 cites W2079891060 @default.
• W2055362838 cites W2085671487 @default.
• W2055362838 cites W2087530336 @default.
• W2055362838 cites W2092293354 @default.
• W2055362838 cites W2097851896 @default.
• W2055362838 cites W2099249565 @default.
• W2055362838 cites W2114967857 @default.
• W2055362838 cites W2115154324 @default.
• W2055362838 cites W2116718309 @default.
• W2055362838 cites W2116869556 @default.
• W2055362838 cites W2121417064 @default.
• W2055362838 cites W2122683945 @default.
• W2055362838 cites W2139122477 @default.
• W2055362838 cites W2156799279 @default.
• W2055362838 cites W2159555560 @default.
• W2055362838 cites W2161224111 @default.
• W2055362838 cites W2161338125 @default.
• W2055362838 cites W2162876548 @default.
• W2055362838 cites W2166729051 @default.
• W2055362838 cites W2172089648 @default.
• W2055362838 cites W2183761009 @default.
• W2055362838 cites W4236225313 @default.
• W2055362838 cites W4247333492 @default.
• W2055362838 doi "https://doi.org/10.1016/j.visres.2010.09.022" @default.
• W2055362838 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3024446" @default.
• W2055362838 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20875442" @default.
• W2055362838 hasPublicationYear "2011" @default.
• W2055362838 type Work @default.
• W2055362838 sameAs 2055362838 @default.
• W2055362838 citedByCount "26" @default.
• W2055362838 countsByYear W20553628382012 @default.
• W2055362838 countsByYear W20553628382013 @default.
• W2055362838 countsByYear W20553628382014 @default.
• W2055362838 countsByYear W20553628382015 @default.
• W2055362838 countsByYear W20553628382016 @default.
• W2055362838 countsByYear W20553628382018 @default.
• W2055362838 countsByYear W20553628382019 @default.
• W2055362838 countsByYear W20553628382020 @default.
• W2055362838 countsByYear W20553628382022 @default.
• W2055362838 countsByYear W20553628382023 @default.
• W2055362838 crossrefType "journal-article" @default.
• W2055362838 hasAuthorship W2055362838A5030506046 @default.
• W2055362838 hasAuthorship W2055362838A5043109858 @default.
• W2055362838 hasAuthorship W2055362838A5083470381 @default.
• W2055362838 hasBestOaLocation W20553628381 @default.

• next