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- W2055415234 abstract "A recombinant yeast strain was constructed of which the cell wall porosity could be reversibly and conditionally modulated. This strain expresses the Nicotiana plumbaginifolia beta-(1,3)-glucanase under control of the Saccharomyces cerevisiae GAL1 promoter and the mating factor alpha 1 prepro-sequence. The following phenotypic effects were observed after expression of this enzyme: (a) expressed beta-(1,3)-glucanase is toxic to the producing yeast cells, which is reflected by a strong growth inhibition; as beta-(1,3)-glucanase could be detected only inside the cells, it seems to interfere with cell wall growth from within the cell; (b) after induction of glucanase, the recombinant strain lost up to 20% of some periplasmic enzymes, as evidenced by the release of normally periplasmic-associated invertase; (c) preliminary growth in a synthetic medium containing galactose significantly increased the transformation efficiency of the recombinant yeast strain." @default.
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- W2055415234 date "1993-02-01" @default.
- W2055415234 modified "2023-09-26" @default.
- W2055415234 title "Phenotypic effects in Saccharomyces cerevisiae after regulated expression of β-(1,3)-glucanase from Nicotiana plumbaginifolia" @default.
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- W2055415234 doi "https://doi.org/10.1016/0168-1656(93)90092-2" @default.
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