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- W2055464325 abstract "A procedure is described for the detection of 17β-19-nortestosterone (17β-19-NT) and 17α-methyltestosterone (17α-MT) in muscle tissue by a combination of high-performance liquid chromatography and radioimmunoassay. The steroids were released from the muscle tissue by enzymic digestion, and the extracts were purified by solid-phase extraction. A meat sample from the retail trade, which was proved to contain 17β-19-NT and 17α-MT residues by gas chromatography—mass spectrometry, and blank meat samples obtained from non-treated experimental animals were purified and the extracts injected into the liquid chromatograph. With an automatic fraction collector, fifteen fractions of 1 ml each were obtained, which after evaporation were subjected to a radioimmunoassay for the steroid concerned. It was demonstrated that none of the fractions from the blank meat samples contains any substance that interfered with the immunochemical detection system. In addition, a good qualitative agreement between the two sets of results was obtained. Although the sample preparation step is rather labour-intensive, the method can be successfully applied as a reliable confirmation method for positive radioimmunoassay screening results in circumstances where no gas chromatography—mass spectrometry facilities are available." @default.
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- W2055464325 date "1991-04-01" @default.
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- W2055464325 title "Combined high-performance liquid chromatography and radioimmunoassay for the screening of 19-nortestosterone and methyltestosterone residues in meat samples" @default.
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- W2055464325 doi "https://doi.org/10.1016/0378-4347(91)80512-b" @default.
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