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- W2055474133 abstract "Rat peripheral blood leukocytes were fractionated into 5–9 subpopulations by centrifugation on discontinuous density gradients of bovine serum albumin. Responses of the various fractions to phytohemagglutinin (PHA) in vitro were compared with their responses to alloantigens in the mixed lymphocyte interaction in vitro and in a graft versus host reaction in vivo. The results showed that: (a) Cells from each of the gradient fractions responded to alloantigens both in vitro and in vivo, (b) Only cells of intermediate density responded vigorously to PHA at a concentration which optimally stimulated unfractionated cells, (c) Low density lymphocytes could be stimulated by 3–9-fold lower concentrations of mitogen. (d) Cells from low and high density fractions, which alone responded poorly to PHA, showed enhanced responses when mixed. These findings may have a significant bearing on the use of the in vitro response to PHA for evaluating the overall function of thymus derived cells in clinically related studies." @default.
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- W2055474133 date "1973-08-01" @default.
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- W2055474133 title "Comparison of the responses of density gradient separated lymphocytes to phytohemagglutinin and histocompatibility antigens" @default.
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- W2055474133 doi "https://doi.org/10.1016/0008-8749(73)90110-x" @default.
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