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• W2055498718 startingPage "311" @default.
• W2055498718 abstract "Phloem is a conductive tissue that allocates nutrients from mature source leaves to sinks such as young developing tissues. Phloem also delivers proteins and RNA species, such as small RNAs and mRNAs. Intensive studies on plant systemic signaling revealed the essential roles of proteins and RNA species. However, many of their functions are still largely unknown, with the roles of transported mRNAs being particularly poorly understood. A major difficulty is the absence of an accurate and comprehensive list of mobile transcripts. In this study, we used a hetero-graft system with Nicotiana benthamiana as the recipient scion and Arabidopsis as the donor stock, to identify transcripts that moved long distances across the graft union. We identified 138 Arabidopsis transcripts as mobile mRNAs, which we collectively termed the mRNA mobilome. Reverse transcription–PCR, quantitative real-time PCR and droplet digital PCR analyses confirmed the mobility. The transcripts included potential signaling factors and, unexpectedly, more general factors. In our investigations, we found no preferred transcript length, no previously known sequence motifs in promoter or transcript sequences and no similarities between the level of the transcripts and that in the source leaves. Grafting experiments regarding the function of ERECTA, an identified transcript, showed that no function of the transcript mobilized. To our knowledge, this is the first report identifying transcripts that move over long distances using a hetero-graft system between different plant taxa." @default.
• W2055498718 created "2016-06-24" @default.
• W2055498718 creator A5012187265 @default.
• W2055498718 creator A5013995574 @default.
• W2055498718 creator A5086543319 @default.
• W2055498718 date "2014-12-19" @default.
• W2055498718 modified "2023-10-13" @default.
• W2055498718 title "Identification of mRNAs that Move Over Long Distances Using an RNA-Seq Analysis of Arabidopsis/Nicotiana benthamiana Heterografts" @default.
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• W2055498718 doi "https://doi.org/10.1093/pcp/pcu210" @default.
• W2055498718 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25527829" @default.
• W2055498718 hasPublicationYear "2014" @default.
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