Matches in SemOpenAlex for { <https://semopenalex.org/work/W2055542910> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2055542910 endingPage "420" @default.
- W2055542910 startingPage "414" @default.
- W2055542910 abstract "The release of vasodilating substances from the vascular endothelium has been postulated to depend on a rise in the level of intracellular free calcium (Cai++). We measured Cai++ in intact monolayers of calf endothelial cells, grown in culture, that were loaded with the fluorescent calcium indicator quin 2. Fluorescence (excitation wavelength 340 nm, emission wavelength 492 nm) was calibrated by raising Cai++ to a maximum with the calcium ionophore ionomycin (0.1 μM) and by lowering it to a minimum with ionomycin plus manganese (0.4 mM), which quenches quin 2 fluorescence completely. Loss of fluorescent dye from the cells was calculated from fluorescence at the isosbestic excitation wavelength (365 nm). Resting Cai++ was 71 ± 3 (SEM) nM. ATP (adenosine -5′-triphosphate) raised Cai++ dose-dependently and reversibly to 458 ± 60 nM at a concentration of 10 μM, and at 0.1 mM to values close to those that occurred under ionomycin. ADP (A-5′-PP) and AMP (A-5′-P) had smaller effects with a maximal Cai++ of 287 ± 72 nM at 30 μM ADP and 176 ± 17 nM at 0.1 mM AMP. At these concentrations, ADP and AMP attenuated significantly the increase of Cai++ under ATP (10 μM). Adenosine (0.1 or 0.3 mM) and acetylcholine (0.1 to 30 μM) enhanced Cai++ inconsistently, by a maximum of 50 nM. These effects were abolished by theophylline and atropine, respectively. In the absence of extracellular calcium, ATP still raised Cai++, although endothelial responsiveness declined after repetitive stimulations. We conclude that activation of purinergic receptors increases intracellular free calcium in endothelial cells, and that this increase is probably an essential trigger for synthesis of prostacyclin and the labile endothelium-derived relaxant factor." @default.
- W2055542910 created "2016-06-24" @default.
- W2055542910 creator A5050471583 @default.
- W2055542910 creator A5053136301 @default.
- W2055542910 date "1986-03-01" @default.
- W2055542910 modified "2023-09-26" @default.
- W2055542910 title "Increased free calcium in endothelial cells under stimulation with adenine nucleotides" @default.
- W2055542910 cites W1561886676 @default.
- W2055542910 cites W1965951774 @default.
- W2055542910 cites W1978778326 @default.
- W2055542910 cites W2020719528 @default.
- W2055542910 cites W2025185754 @default.
- W2055542910 cites W2039923774 @default.
- W2055542910 cites W2045594638 @default.
- W2055542910 cites W2065705900 @default.
- W2055542910 cites W2067814812 @default.
- W2055542910 cites W2068514404 @default.
- W2055542910 cites W2072566202 @default.
- W2055542910 cites W2109799410 @default.
- W2055542910 cites W2113943014 @default.
- W2055542910 cites W2115508204 @default.
- W2055542910 cites W2130503561 @default.
- W2055542910 cites W2132184473 @default.
- W2055542910 cites W2141203325 @default.
- W2055542910 cites W2169182236 @default.
- W2055542910 cites W2239854841 @default.
- W2055542910 cites W4210963323 @default.
- W2055542910 doi "https://doi.org/10.1002/jcp.1041260312" @default.
- W2055542910 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3949890" @default.
- W2055542910 hasPublicationYear "1986" @default.
- W2055542910 type Work @default.
- W2055542910 sameAs 2055542910 @default.
- W2055542910 citedByCount "167" @default.
- W2055542910 countsByYear W20555429102013 @default.
- W2055542910 countsByYear W20555429102015 @default.
- W2055542910 countsByYear W20555429102016 @default.
- W2055542910 countsByYear W20555429102022 @default.
- W2055542910 crossrefType "journal-article" @default.
- W2055542910 hasAuthorship W2055542910A5050471583 @default.
- W2055542910 hasAuthorship W2055542910A5053136301 @default.
- W2055542910 hasConcept C121332964 @default.
- W2055542910 hasConcept C12554922 @default.
- W2055542910 hasConcept C134018914 @default.
- W2055542910 hasConcept C178790620 @default.
- W2055542910 hasConcept C181080969 @default.
- W2055542910 hasConcept C185592680 @default.
- W2055542910 hasConcept C24998067 @default.
- W2055542910 hasConcept C2776989580 @default.
- W2055542910 hasConcept C2776991684 @default.
- W2055542910 hasConcept C2778626300 @default.
- W2055542910 hasConcept C28406088 @default.
- W2055542910 hasConcept C2986317502 @default.
- W2055542910 hasConcept C519063684 @default.
- W2055542910 hasConcept C55493867 @default.
- W2055542910 hasConcept C62520636 @default.
- W2055542910 hasConcept C86803240 @default.
- W2055542910 hasConcept C91881484 @default.
- W2055542910 hasConceptScore W2055542910C121332964 @default.
- W2055542910 hasConceptScore W2055542910C12554922 @default.
- W2055542910 hasConceptScore W2055542910C134018914 @default.
- W2055542910 hasConceptScore W2055542910C178790620 @default.
- W2055542910 hasConceptScore W2055542910C181080969 @default.
- W2055542910 hasConceptScore W2055542910C185592680 @default.
- W2055542910 hasConceptScore W2055542910C24998067 @default.
- W2055542910 hasConceptScore W2055542910C2776989580 @default.
- W2055542910 hasConceptScore W2055542910C2776991684 @default.
- W2055542910 hasConceptScore W2055542910C2778626300 @default.
- W2055542910 hasConceptScore W2055542910C28406088 @default.
- W2055542910 hasConceptScore W2055542910C2986317502 @default.
- W2055542910 hasConceptScore W2055542910C519063684 @default.
- W2055542910 hasConceptScore W2055542910C55493867 @default.
- W2055542910 hasConceptScore W2055542910C62520636 @default.
- W2055542910 hasConceptScore W2055542910C86803240 @default.
- W2055542910 hasConceptScore W2055542910C91881484 @default.
- W2055542910 hasIssue "3" @default.
- W2055542910 hasLocation W20555429101 @default.
- W2055542910 hasLocation W20555429102 @default.
- W2055542910 hasOpenAccess W2055542910 @default.
- W2055542910 hasPrimaryLocation W20555429101 @default.
- W2055542910 hasRelatedWork W1765098545 @default.
- W2055542910 hasRelatedWork W2014282608 @default.
- W2055542910 hasRelatedWork W2015506378 @default.
- W2055542910 hasRelatedWork W202132418 @default.
- W2055542910 hasRelatedWork W2042521886 @default.
- W2055542910 hasRelatedWork W2052785990 @default.
- W2055542910 hasRelatedWork W2096400641 @default.
- W2055542910 hasRelatedWork W2130212418 @default.
- W2055542910 hasRelatedWork W2253095283 @default.
- W2055542910 hasRelatedWork W83059117 @default.
- W2055542910 hasVolume "126" @default.
- W2055542910 isParatext "false" @default.
- W2055542910 isRetracted "false" @default.
- W2055542910 magId "2055542910" @default.
- W2055542910 workType "article" @default.