FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2055563705> ?p ?o ?g. }

• W2055563705 endingPage "1355" @default.
• W2055563705 startingPage "1349" @default.
• W2055563705 abstract "DNA–protein conjugates have frequently been used as versatile molecular tools for a variety of applications in biotechnology to harness synergistic effects of DNA and protein functions. With applications for DNA–protein conjugates growing, easy-to-use and economical methods for the synthesis of DNA–protein conjugates are required. In this study, we developed a method for site-specific labeling of single-stranded DNA (ssDNA) to a recombinant protein of interest (POI) through the Gene-A* protein (Gene-A*) from bacteriophage phi X174, without any chemical modifications of ssDNA. Gene-A* protein is an enzyme that site-selectively cleaves an oligodeoxyribonucleotide (ODN) containing a Gene-A* recognition sequence, at which point a tyrosine residue of Gene-A* is bonded to the 5′-phosphoryl group of the cleavage site via a stable phosphotyrosine linkage. Here, we constructed three kinds of recombinant proteins fused to Gene-A*: N-terminally Gene-A*-fused enhanced green fluorescent protein (EGFP), C-terminally Gene-A*-fused EGFP, and N-terminally Gene-A*-fused firefly luciferase (FLuc). The reaction yields of DNA–protein conjugation catalyzed by the Gene-A* moiety reached 80–90% in the three proteins, and kinetic study revealed that the reaction achieved a steady state after 10 min. Moreover, dot blot analyses were performed to evaluate the hybridization and aptamer-forming ability of ssDNA conjugated to the Gene-A* moiety of a recombinant Gene-A*-FLuc protein. This study demonstrated that a strategy using recombinant proteins fused to Gene-A* could offer a versatile, rapid, easy-to-use, and economical platform for producing DNA–protein conjugates." @default.
• W2055563705 created "2016-06-24" @default.
• W2055563705 creator A5039689405 @default.
• W2055563705 creator A5042045030 @default.
• W2055563705 creator A5062352012 @default.
• W2055563705 creator A5076745722 @default.
• W2055563705 date "2012-06-01" @default.
• W2055563705 modified "2023-09-23" @default.
• W2055563705 title "Construction of Semisynthetic DNA–Protein Conjugates with Phi X174 Gene-A* Protein" @default.
• W2055563705 cites W1543188542 @default.
• W2055563705 cites W1855917743 @default.
• W2055563705 cites W1964858690 @default.
• W2055563705 cites W1975209319 @default.
• W2055563705 cites W1980594598 @default.
• W2055563705 cites W1991473377 @default.
• W2055563705 cites W2004901112 @default.
• W2055563705 cites W2005615084 @default.
• W2055563705 cites W2011436554 @default.
• W2055563705 cites W2016689148 @default.
• W2055563705 cites W2024086291 @default.
• W2055563705 cites W2024700861 @default.
• W2055563705 cites W2027867184 @default.
• W2055563705 cites W2028254012 @default.
• W2055563705 cites W2033647473 @default.
• W2055563705 cites W2046090919 @default.
• W2055563705 cites W2046765343 @default.
• W2055563705 cites W2047786995 @default.
• W2055563705 cites W2051667191 @default.
• W2055563705 cites W2053590462 @default.
• W2055563705 cites W2057788156 @default.
• W2055563705 cites W2073406929 @default.
• W2055563705 cites W2082572792 @default.
• W2055563705 cites W2082654411 @default.
• W2055563705 cites W2082984134 @default.
• W2055563705 cites W2087670364 @default.
• W2055563705 cites W2088885969 @default.
• W2055563705 cites W2095235261 @default.
• W2055563705 cites W2095886160 @default.
• W2055563705 cites W2112239422 @default.
• W2055563705 cites W2116245038 @default.
• W2055563705 cites W2156578843 @default.
• W2055563705 cites W2161027891 @default.
• W2055563705 cites W2171446065 @default.
• W2055563705 doi "https://doi.org/10.1021/bc300118m" @default.
• W2055563705 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22616938" @default.
• W2055563705 hasPublicationYear "2012" @default.
• W2055563705 type Work @default.
• W2055563705 sameAs 2055563705 @default.
• W2055563705 citedByCount "15" @default.
• W2055563705 countsByYear W20555637052013 @default.
• W2055563705 countsByYear W20555637052014 @default.
• W2055563705 countsByYear W20555637052015 @default.
• W2055563705 countsByYear W20555637052017 @default.
• W2055563705 countsByYear W20555637052018 @default.
• W2055563705 countsByYear W20555637052019 @default.
• W2055563705 countsByYear W20555637052020 @default.
• W2055563705 countsByYear W20555637052021 @default.
• W2055563705 countsByYear W20555637052022 @default.
• W2055563705 crossrefType "journal-article" @default.
• W2055563705 hasAuthorship W2055563705A5039689405 @default.
• W2055563705 hasAuthorship W2055563705A5042045030 @default.
• W2055563705 hasAuthorship W2055563705A5062352012 @default.
• W2055563705 hasAuthorship W2055563705A5076745722 @default.
• W2055563705 hasConcept C104317684 @default.
• W2055563705 hasConcept C116010114 @default.
• W2055563705 hasConcept C119113125 @default.
• W2055563705 hasConcept C123894998 @default.
• W2055563705 hasConcept C136071829 @default.
• W2055563705 hasConcept C153911025 @default.
• W2055563705 hasConcept C167625842 @default.
• W2055563705 hasConcept C185592680 @default.
• W2055563705 hasConcept C40767141 @default.
• W2055563705 hasConcept C552990157 @default.
• W2055563705 hasConcept C55493867 @default.
• W2055563705 hasConcept C86803240 @default.
• W2055563705 hasConceptScore W2055563705C104317684 @default.
• W2055563705 hasConceptScore W2055563705C116010114 @default.
• W2055563705 hasConceptScore W2055563705C119113125 @default.
• W2055563705 hasConceptScore W2055563705C123894998 @default.
• W2055563705 hasConceptScore W2055563705C136071829 @default.
• W2055563705 hasConceptScore W2055563705C153911025 @default.
• W2055563705 hasConceptScore W2055563705C167625842 @default.
• W2055563705 hasConceptScore W2055563705C185592680 @default.
• W2055563705 hasConceptScore W2055563705C40767141 @default.
• W2055563705 hasConceptScore W2055563705C552990157 @default.
• W2055563705 hasConceptScore W2055563705C55493867 @default.
• W2055563705 hasConceptScore W2055563705C86803240 @default.
• W2055563705 hasIssue "6" @default.
• W2055563705 hasLocation W20555637051 @default.
• W2055563705 hasLocation W20555637052 @default.
• W2055563705 hasOpenAccess W2055563705 @default.
• W2055563705 hasPrimaryLocation W20555637051 @default.
• W2055563705 hasRelatedWork W1432577577 @default.
• W2055563705 hasRelatedWork W2122212633 @default.
• W2055563705 hasRelatedWork W2350532293 @default.
• W2055563705 hasRelatedWork W2357972350 @default.
• W2055563705 hasRelatedWork W2371993564 @default.
• W2055563705 hasRelatedWork W2381845779 @default.

• next