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- W2055583485 abstract "The introduction of 2'-deoxyuridine 5'-monophosphate and its analog, 5-fluoro-2'-deoxyuridine 5'-monophosphate, into intact CCRF-CEM and NIH3T3 cells was achieved by electroporation. Following electroporation, cells were shown to be fully functional as monitored by the incorporation of deoxyuridylate, after conversion to thymidylate, into DNA. Pretreatment of cells with fluorodeoxyuridine completely abolished this effect. In contrast, introduction of the fluoro analog into cells by electroporation markedly inhibited both DNA synthesis and cell growth in a time-dependent manner. Thus, electroporation offers a powerful tool to permeabilize cells to a variety of cellular metabolites and antimetabolites." @default.
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- W2055583485 date "1987-04-01" @default.
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- W2055583485 title "Use of electroporation to study the cytotoxic effects of fluorodeoxyuridylate in intact cells" @default.
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- W2055583485 doi "https://doi.org/10.1016/0006-2952(87)90092-x" @default.
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