Matches in SemOpenAlex for { <https://semopenalex.org/work/W2055657997> ?p ?o ?g. }
- W2055657997 endingPage "123" @default.
- W2055657997 startingPage "113" @default.
- W2055657997 abstract "The metabolism of [1-14C]arachidonic acid by microsomal and purified prostaglandin (PG) H synthase was investigated. HPLC analysis confirmed that arachidonic acid (20:4) was extensively converted into prostaglandin G2 (PGG2) and/or prostaglandin H2 (PGH2) but several minor labelled products were formed in addition. Their formation, mediated by PGH synthase was established by inhibition with aspirin and indomethacin [Hecker, M., Hatzelmann, A. & Ullrich, V. (1987) Biochem. Pharmacol. 36, 851–855]. Upon comparison with authentic reference material these unknown PGH synthase metabolites were identified with respect to chromatographic properties, ultraviolet spectroscopy and mass spectrometry as 11 (R)-hydroperoxy-5Z,8Z,12E,14Z-eicosatetraenoic acid (11-OOH-20:4), 12(S)-hydroperoxy-5Z,8E,10E-heptadecatrienoic acid (OOH-17:3), 12(S)-hydroxy-5Z,8E,10E-heptadecatrienoic acid (OH-17:3), 15(RS)-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid (15-OOH-20:4), 15(RS)-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid (15-OH-20:4), 13-hydroxy-5Z,14Z-prostaglandin H2, 15(S)-hydroxy-8-iso-5Z,13E-prostaglandin H2 and 15-oxo-prostaglandin H2. Unlike PGG2 and PGH2, 8-iso-PGH2, 13-hydroxy-PGH2 and 15-oxo-PGH2 failed to induce aggregation of washed human platelets and to form thromboxane upon incubation with homogeneous human platelet thromboxane synthase. In contrast to the formation of OOH-17:3, 15-oxo-PGH2 and OH-17:3 which can be attributed to the heme-catalyzed decomposition of PGG2 and PGH2, 11-OOH-20:4, 15-(O)OH-20:4-, 8 iso-PGH2 and 13-hydroxy-PGH2 represent potential side products of arachidonic acid conversion into PG endoperoxides. Their formation allows to conclude on PGH synthase mechanism and its intermediates for which an extended reaction scheme is proposed." @default.
- W2055657997 created "2016-06-24" @default.
- W2055657997 creator A5036354220 @default.
- W2055657997 creator A5063375455 @default.
- W2055657997 creator A5068069545 @default.
- W2055657997 creator A5069878962 @default.
- W2055657997 date "1987-11-01" @default.
- W2055657997 modified "2023-09-29" @default.
- W2055657997 title "Identification of novel arachidonic acid metabolites formed by prostaglandin H synthase" @default.
- W2055657997 cites W1187643695 @default.
- W2055657997 cites W1496885000 @default.
- W2055657997 cites W1514733921 @default.
- W2055657997 cites W1518564255 @default.
- W2055657997 cites W1522779323 @default.
- W2055657997 cites W1531198291 @default.
- W2055657997 cites W1531513729 @default.
- W2055657997 cites W1543173789 @default.
- W2055657997 cites W1543274520 @default.
- W2055657997 cites W1555140230 @default.
- W2055657997 cites W1585808802 @default.
- W2055657997 cites W1594115484 @default.
- W2055657997 cites W1659584317 @default.
- W2055657997 cites W1964649455 @default.
- W2055657997 cites W1966244893 @default.
- W2055657997 cites W1971670768 @default.
- W2055657997 cites W1973523751 @default.
- W2055657997 cites W1981770747 @default.
- W2055657997 cites W1995969948 @default.
- W2055657997 cites W2006329809 @default.
- W2055657997 cites W2008574207 @default.
- W2055657997 cites W2019607384 @default.
- W2055657997 cites W2024297591 @default.
- W2055657997 cites W2027352121 @default.
- W2055657997 cites W2038229413 @default.
- W2055657997 cites W2045236813 @default.
- W2055657997 cites W2050568232 @default.
- W2055657997 cites W2056802836 @default.
- W2055657997 cites W2059959435 @default.
- W2055657997 cites W2068707504 @default.
- W2055657997 cites W2070784942 @default.
- W2055657997 cites W2076367727 @default.
- W2055657997 cites W2076674649 @default.
- W2055657997 cites W2085784281 @default.
- W2055657997 cites W2107569707 @default.
- W2055657997 cites W2131066517 @default.
- W2055657997 cites W2156116756 @default.
- W2055657997 cites W2171888981 @default.
- W2055657997 cites W2328344636 @default.
- W2055657997 cites W3023954712 @default.
- W2055657997 cites W4239674272 @default.
- W2055657997 cites W989142126 @default.
- W2055657997 doi "https://doi.org/10.1111/j.1432-1033.1987.tb13587.x" @default.
- W2055657997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3119336" @default.
- W2055657997 hasPublicationYear "1987" @default.
- W2055657997 type Work @default.
- W2055657997 sameAs 2055657997 @default.
- W2055657997 citedByCount "62" @default.
- W2055657997 countsByYear W20556579972012 @default.
- W2055657997 countsByYear W20556579972013 @default.
- W2055657997 countsByYear W20556579972015 @default.
- W2055657997 countsByYear W20556579972016 @default.
- W2055657997 countsByYear W20556579972017 @default.
- W2055657997 countsByYear W20556579972018 @default.
- W2055657997 countsByYear W20556579972019 @default.
- W2055657997 countsByYear W20556579972020 @default.
- W2055657997 countsByYear W20556579972021 @default.
- W2055657997 countsByYear W20556579972023 @default.
- W2055657997 crossrefType "journal-article" @default.
- W2055657997 hasAuthorship W2055657997A5036354220 @default.
- W2055657997 hasAuthorship W2055657997A5063375455 @default.
- W2055657997 hasAuthorship W2055657997A5068069545 @default.
- W2055657997 hasAuthorship W2055657997A5069878962 @default.
- W2055657997 hasBestOaLocation W20556579971 @default.
- W2055657997 hasConcept C112243037 @default.
- W2055657997 hasConcept C126322002 @default.
- W2055657997 hasConcept C170493617 @default.
- W2055657997 hasConcept C173202427 @default.
- W2055657997 hasConcept C181199279 @default.
- W2055657997 hasConcept C185592680 @default.
- W2055657997 hasConcept C2776785769 @default.
- W2055657997 hasConcept C2777361909 @default.
- W2055657997 hasConcept C2778078955 @default.
- W2055657997 hasConcept C2780664492 @default.
- W2055657997 hasConcept C2780978440 @default.
- W2055657997 hasConcept C2781024287 @default.
- W2055657997 hasConcept C55493867 @default.
- W2055657997 hasConcept C71240020 @default.
- W2055657997 hasConcept C71924100 @default.
- W2055657997 hasConcept C89560881 @default.
- W2055657997 hasConceptScore W2055657997C112243037 @default.
- W2055657997 hasConceptScore W2055657997C126322002 @default.
- W2055657997 hasConceptScore W2055657997C170493617 @default.
- W2055657997 hasConceptScore W2055657997C173202427 @default.
- W2055657997 hasConceptScore W2055657997C181199279 @default.
- W2055657997 hasConceptScore W2055657997C185592680 @default.
- W2055657997 hasConceptScore W2055657997C2776785769 @default.
- W2055657997 hasConceptScore W2055657997C2777361909 @default.
- W2055657997 hasConceptScore W2055657997C2778078955 @default.