Matches in SemOpenAlex for { <https://semopenalex.org/work/W2055709849> ?p ?o ?g. }
- W2055709849 endingPage "8347" @default.
- W2055709849 startingPage "8331" @default.
- W2055709849 abstract "The CCR4-NOT complex, the major deadenylase in eukaryotes, plays crucial roles in gene expression at the levels of transcription, mRNA decay, and protein degradation. GW182/TNRC6 proteins, which are core components of the microRNA-induced silencing complex in animals, stimulate deadenylation and repress translation via recruitment of the CCR4-NOT complex. Here we report a heterologous experimental system that recapitulates the recruitment of CCR4-NOT complex by TNRC6 in S. cerevisiae. Using this system, we characterize conserved functions of the CCR4-NOT complex. The complex stimulates degradation of mRNA from the 5′ end by Xrn1, in a manner independent of both translation and deadenylation. This degradation pathway is probably conserved in miRNA-mediated gene silencing in zebrafish. Furthermore, the mRNA fate modulators Dhh1 and Pat1 redundantly stimulate mRNA decay, but both factors are required for poly(A) tail-independent translation repression by tethered TNRC6A. Our tethering-based reconstitution system reveals that the conserved architecture of Not1/CNOT1 provides a binding surface for TNRC6, thereby connecting microRNA-induced silencing complex to the decapping machinery as well as the translation apparatus." @default.
- W2055709849 created "2016-06-24" @default.
- W2055709849 creator A5004901799 @default.
- W2055709849 creator A5022850583 @default.
- W2055709849 creator A5052409368 @default.
- W2055709849 creator A5061533640 @default.
- W2055709849 date "2015-03-01" @default.
- W2055709849 modified "2023-10-13" @default.
- W2055709849 title "Roles of mRNA Fate Modulators Dhh1 and Pat1 in TNRC6-dependent Gene Silencing Recapitulated in Yeast" @default.
- W2055709849 cites W1499220571 @default.
- W2055709849 cites W1964297281 @default.
- W2055709849 cites W1965677186 @default.
- W2055709849 cites W1969019156 @default.
- W2055709849 cites W1970390320 @default.
- W2055709849 cites W1974005002 @default.
- W2055709849 cites W1978337436 @default.
- W2055709849 cites W1988249075 @default.
- W2055709849 cites W1996157637 @default.
- W2055709849 cites W1998128193 @default.
- W2055709849 cites W2000183154 @default.
- W2055709849 cites W2000456081 @default.
- W2055709849 cites W2003322154 @default.
- W2055709849 cites W2004864449 @default.
- W2055709849 cites W2005300549 @default.
- W2055709849 cites W2005433767 @default.
- W2055709849 cites W2007639680 @default.
- W2055709849 cites W2010461414 @default.
- W2055709849 cites W2021800473 @default.
- W2055709849 cites W2022832110 @default.
- W2055709849 cites W2026570758 @default.
- W2055709849 cites W2026945839 @default.
- W2055709849 cites W2028583641 @default.
- W2055709849 cites W2030473151 @default.
- W2055709849 cites W2045810016 @default.
- W2055709849 cites W2048443894 @default.
- W2055709849 cites W2049868101 @default.
- W2055709849 cites W2050362152 @default.
- W2055709849 cites W2053227231 @default.
- W2055709849 cites W2057532472 @default.
- W2055709849 cites W2057604708 @default.
- W2055709849 cites W2063877028 @default.
- W2055709849 cites W2064298041 @default.
- W2055709849 cites W2067024190 @default.
- W2055709849 cites W2074675420 @default.
- W2055709849 cites W2076984671 @default.
- W2055709849 cites W2077584262 @default.
- W2055709849 cites W2078277064 @default.
- W2055709849 cites W2080182910 @default.
- W2055709849 cites W2084905877 @default.
- W2055709849 cites W2085519695 @default.
- W2055709849 cites W2088215815 @default.
- W2055709849 cites W2088399865 @default.
- W2055709849 cites W2091544975 @default.
- W2055709849 cites W2096688350 @default.
- W2055709849 cites W2099383089 @default.
- W2055709849 cites W2105992602 @default.
- W2055709849 cites W2108888759 @default.
- W2055709849 cites W2136400757 @default.
- W2055709849 cites W2141287804 @default.
- W2055709849 cites W2141325721 @default.
- W2055709849 cites W2142571092 @default.
- W2055709849 cites W2144795764 @default.
- W2055709849 cites W2147114301 @default.
- W2055709849 cites W2148916879 @default.
- W2055709849 cites W2151069484 @default.
- W2055709849 cites W2152946647 @default.
- W2055709849 cites W2153974400 @default.
- W2055709849 cites W2154709300 @default.
- W2055709849 cites W2158010566 @default.
- W2055709849 cites W2158584001 @default.
- W2055709849 cites W2159638567 @default.
- W2055709849 cites W2161217765 @default.
- W2055709849 cites W2161735420 @default.
- W2055709849 cites W2163106706 @default.
- W2055709849 cites W2167144689 @default.
- W2055709849 cites W2169590997 @default.
- W2055709849 doi "https://doi.org/10.1074/jbc.m114.615088" @default.
- W2055709849 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4375487" @default.
- W2055709849 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25657010" @default.
- W2055709849 hasPublicationYear "2015" @default.
- W2055709849 type Work @default.
- W2055709849 sameAs 2055709849 @default.
- W2055709849 citedByCount "7" @default.
- W2055709849 countsByYear W20557098492015 @default.
- W2055709849 countsByYear W20557098492016 @default.
- W2055709849 countsByYear W20557098492017 @default.
- W2055709849 countsByYear W20557098492022 @default.
- W2055709849 crossrefType "journal-article" @default.
- W2055709849 hasAuthorship W2055709849A5004901799 @default.
- W2055709849 hasAuthorship W2055709849A5022850583 @default.
- W2055709849 hasAuthorship W2055709849A5052409368 @default.
- W2055709849 hasAuthorship W2055709849A5061533640 @default.
- W2055709849 hasBestOaLocation W20557098491 @default.
- W2055709849 hasConcept C104317684 @default.
- W2055709849 hasConcept C105580179 @default.
- W2055709849 hasConcept C119056186 @default.
- W2055709849 hasConcept C2779222958 @default.
- W2055709849 hasConcept C54355233 @default.