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• W2055804915 abstract "Abstract The isoelectric point of hirudine has been estimated by the isoelectric condensation method of Svensson. Its diffusion constant has been determined using the method of diffusion in agar gel. The molecular weight of hirudine as estimated by its behaviour on Sephadex is only an approximate value; this behaviour is better correlated with the molecular size of the protein and therefore to the Stokes radius and the diffusion constant. The value of this radius calculated from the diffusion constant in fact agrees with that deduced from the ratio V e /V o which is obtained after filtration on Sephadex. The sedimentation constant has been determined by ultracentrifugation in a sucrose gradient. The value of the molecular weight of hirudine calculated in this way is somewhat lower than expected. This may suggest the influence of sucrose on the degree of hydration of the molecule leading to a modification of its density." @default.
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• W2055804915 date "1966-08-24" @default.
• W2055804915 modified "2023-09-23" @default.
• W2055804915 title "[Study of certain physicochemical constants of hirudin]." @default.
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