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- W2055830898 abstract "Intraparenchymal injection of a viral vector enables conditional gene manipulation in distinct populations of neurons or particular regions of the central nervous system. We demonstrate a stereotaxic injection technique that allows targeted gene expression or silencing in the dorsal horn of the mouse spinal cord. The surgical procedure is brief. It requires laminectomy of a single vertebra, providing for quick recovery of the animal and unimpaired motility of the spine. Controlled injection of a small vector suspension volume at low speed and use of a microsyringe with beveled glass cannula minimize the tissue lesion. The local immune response to the vector depends on the intrinsic properties of the virus employed; in our experience, it is minor and short-lived when a recombinant adeno-associated virus is used. A reporter gene such as enhanced green fluorescent protein facilitates monitoring spatial distribution of the vector, and the efficacy and cellular specificity of the transfection." @default.
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- W2055830898 date "2013-03-18" @default.
- W2055830898 modified "2023-09-27" @default.
- W2055830898 title "Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord" @default.
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- W2055830898 doi "https://doi.org/10.3791/50313" @default.
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