FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2055842954> ?p ?o ?g. }

• W2055842954 endingPage "203" @default.
• W2055842954 startingPage "196" @default.
• W2055842954 abstract "The study was designed to evaluate in vitro the cellular mechanisms of the single nucleotide polymorphism (SNP) p.N680S of the FSH receptor gene (FSHR) in human granulosa cells (GC) and included patients homozygous for the FSHR SNP (NN/SS) undergoing ovarian stimulation. GC were isolated during oocyte retrieval and cultured for 1–7 days. Basal oestradiol and progesterone concentrations were measured after short-term culture. The kinetics of cAMP, oestradiol and progesterone concentrations in response to various amounts of FSH were analysed in a 6–7 day culture. Basal oestradiol, but not progesterone, concentrations on day 1 of GC culture, were significantly higher in NN compared with SS (P = 0.045), but non-responsive to FSH stimulation. Immunofluorescence microscopy demonstrated the re-appearance of FSHR expression with increasing days in culture. Upon stimulation with FSH, GC cultured for 6–7 days displayed a dose-dependent increase of cAMP, oestradiol and progesterone but no difference in the EC50 values between both variants. Primary long-term GC cultures are a suitable system to study the effects of FSH in vitro. However, the experiments suggest that factors down-stream of progesterone production or external to GC might be involved in the clinically observed differences in an FSHR variant-mediated response to FSH.The prediction of an individual patient’s response to FSH is essential to optimize treatment outcome for assisted reproductive techniques and to minimize the associated risk of ovarian hyperstimulation syndrome. Previous clinical studies have demonstrated differences in the ovarian response depending on a single nucleotide polymorphism (SNP) at amino acid position 680 of the FSH receptor (FSHR). This study was designed to evaluate in vitro the underlying cellular mechanisms. It included two patient groups homozygous for the two variants of the FSHR SNP 680 (NN or SS) undergoing ovarian stimulation for assisted reproductive techniques. Granulosa cells (GC) as the target of FSH action in the ovary and main source of female sex steroids were isolated during oocyte retrieval and cultured for 1–7 days. Basal concentrations of oestradiol and progesterone were measured after short-term culture and oestradiol, but not progesterone, was significantly higher in the NN compared to the SS variant. As GC were irresponsive to FSH stimulation, the culture period was extended and re-appearance of FSHR expression was demonstrated by immunofluorescence microscopy with increasing days in culture. Upon stimulation with FSH, GC at day 6–7 of culture displayed a dose-dependent increase of cyclic adenosine monophosphate (cAMP), oestradiol and progesterone, but no statistically significant difference in the kinetic values between both FSHR variants. In conclusion, we have established a suitable system to study the effects of FSH ex vivo in primary long-term GC cultures. To fully explain the clinically observed differences in the FSHR variant-mediated response to FSH, factors downstream of P production or external to GC might be involved." @default.
• W2055842954 created "2016-06-24" @default.
• W2055842954 creator A5017423809 @default.
• W2055842954 creator A5020172375 @default.
• W2055842954 creator A5034776080 @default.
• W2055842954 creator A5037889734 @default.
• W2055842954 creator A5048402117 @default.
• W2055842954 creator A5061320191 @default.
• W2055842954 creator A5063086787 @default.
• W2055842954 creator A5086293122 @default.
• W2055842954 creator A5089212670 @default.
• W2055842954 date "2011-08-01" @default.
• W2055842954 modified "2023-09-27" @default.
• W2055842954 title "Effects of the FSH receptor gene polymorphism p.N680S on cAMP and steroid production in cultured primary human granulosa cells" @default.
• W2055842954 cites W1968446974 @default.
• W2055842954 cites W1973393731 @default.
• W2055842954 cites W1990037728 @default.
• W2055842954 cites W1997336128 @default.
• W2055842954 cites W2005095165 @default.
• W2055842954 cites W2005444928 @default.
• W2055842954 cites W2012639148 @default.
• W2055842954 cites W2037881182 @default.
• W2055842954 cites W2041655797 @default.
• W2055842954 cites W2057336811 @default.
• W2055842954 cites W2061917914 @default.
• W2055842954 cites W2063535530 @default.
• W2055842954 cites W2066576538 @default.
• W2055842954 cites W2087342178 @default.
• W2055842954 cites W2090423690 @default.
• W2055842954 cites W2091194437 @default.
• W2055842954 cites W2091468986 @default.
• W2055842954 cites W2103388435 @default.
• W2055842954 cites W2104298130 @default.
• W2055842954 cites W2106487699 @default.
• W2055842954 cites W2113704542 @default.
• W2055842954 cites W2132058254 @default.
• W2055842954 cites W2135613192 @default.
• W2055842954 cites W2147404179 @default.
• W2055842954 cites W2147912605 @default.
• W2055842954 cites W2154910006 @default.
• W2055842954 doi "https://doi.org/10.1016/j.rbmo.2011.04.009" @default.
• W2055842954 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21680247" @default.
• W2055842954 hasPublicationYear "2011" @default.
• W2055842954 type Work @default.
• W2055842954 sameAs 2055842954 @default.
• W2055842954 citedByCount "59" @default.
• W2055842954 countsByYear W20558429542012 @default.
• W2055842954 countsByYear W20558429542013 @default.
• W2055842954 countsByYear W20558429542014 @default.
• W2055842954 countsByYear W20558429542015 @default.
• W2055842954 countsByYear W20558429542016 @default.
• W2055842954 countsByYear W20558429542017 @default.
• W2055842954 countsByYear W20558429542018 @default.
• W2055842954 countsByYear W20558429542019 @default.
• W2055842954 countsByYear W20558429542020 @default.
• W2055842954 countsByYear W20558429542021 @default.
• W2055842954 countsByYear W20558429542022 @default.
• W2055842954 countsByYear W20558429542023 @default.
• W2055842954 crossrefType "journal-article" @default.
• W2055842954 hasAuthorship W2055842954A5017423809 @default.
• W2055842954 hasAuthorship W2055842954A5020172375 @default.
• W2055842954 hasAuthorship W2055842954A5034776080 @default.
• W2055842954 hasAuthorship W2055842954A5037889734 @default.
• W2055842954 hasAuthorship W2055842954A5048402117 @default.
• W2055842954 hasAuthorship W2055842954A5061320191 @default.
• W2055842954 hasAuthorship W2055842954A5063086787 @default.
• W2055842954 hasAuthorship W2055842954A5086293122 @default.
• W2055842954 hasAuthorship W2055842954A5089212670 @default.
• W2055842954 hasBestOaLocation W20558429541 @default.
• W2055842954 hasConcept C104317684 @default.
• W2055842954 hasConcept C121608353 @default.
• W2055842954 hasConcept C126322002 @default.
• W2055842954 hasConcept C134018914 @default.
• W2055842954 hasConcept C135763542 @default.
• W2055842954 hasConcept C139275648 @default.
• W2055842954 hasConcept C153209595 @default.
• W2055842954 hasConcept C16685009 @default.
• W2055842954 hasConcept C170493617 @default.
• W2055842954 hasConcept C196843134 @default.
• W2055842954 hasConcept C24998067 @default.
• W2055842954 hasConcept C2776188440 @default.
• W2055842954 hasConcept C2776690073 @default.
• W2055842954 hasConcept C2777203538 @default.
• W2055842954 hasConcept C2778024521 @default.
• W2055842954 hasConcept C2778324911 @default.
• W2055842954 hasConcept C2778575703 @default.
• W2055842954 hasConcept C2779279165 @default.
• W2055842954 hasConcept C2779306644 @default.
• W2055842954 hasConcept C29390026 @default.
• W2055842954 hasConcept C530470458 @default.
• W2055842954 hasConcept C54355233 @default.
• W2055842954 hasConcept C71315377 @default.
• W2055842954 hasConcept C71924100 @default.
• W2055842954 hasConcept C84606932 @default.
• W2055842954 hasConcept C86803240 @default.
• W2055842954 hasConcept C95444343 @default.
• W2055842954 hasConcept C98717036 @default.
• W2055842954 hasConceptScore W2055842954C104317684 @default.

• next