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• W2055851951 abstract "myo-Inositol is being investigated as a biomarker to monitor disease states involving the central nervous system. We have developed and validated a quantitative method to study endogenous myo-inositol metabolism in rat brain tissue. Tissue samples were homogenized, and their myo-inositol content was determined using spiked calibration curves and mass spectrometry. The assay was validated on an LC/MS/MS platform, and specificity was evaluated using accurate mass measurements. A novel chiral LC/MS/MS method was also developed to resolve myo-inositol from other endogenous inositol epimers and confirm the selectivity of the quantitative procedure. The validated method is selective, convenient, precise (<15% RSD), accurate (<15% RE), and sensitive over a linear range of 0.100−100 μg/mL. This method could potentially be used as an instrument for monitoring pathological conditions related to psychotherapeutics, as well as a tool for screening curative pharmaceuticals for efficacy." @default.
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• W2055851951 date "2004-07-17" @default.
• W2055851951 modified "2023-10-16" @default.
• W2055851951 title "Development and Validation of an LC/MS/MS Procedure for the Quantification of Endogenous <i>myo</i>-Inositol Concentrations in Rat Brain Tissue Homogenates" @default.
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• W2055851951 doi "https://doi.org/10.1021/ac049746w" @default.
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