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- W2055876613 abstract "The human ADH5 gene was reported to lack the last exon compared to other mammalian ADHs and consequently should be expressed as a truncated protein. Here we show with PCR amplification of 3'-cDNA ends that the ADH5 gene harbors the missing exon. Besides a cDNA identical to the published sequence, we found full-length transcripts that contained additional codons for eight amino acid residues. Northern blot analysis established the full-length variant as the major transcript with the strongest signal from adult liver. Sequence analysis of genomic DNA confirmed that the ADH5 gene displays composite internal/terminal exons, which can be differentially processed; i.e., 3'-end generation is a result of competition between polyadenylation and splicing." @default.
- W2055876613 created "2016-06-24" @default.
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- W2055876613 date "2000-11-01" @default.
- W2055876613 modified "2023-10-02" @default.
- W2055876613 title "Human Class V Alcohol Dehydrogenase (ADH5): A Complex Transcription Unit Generates C-Terminal Multiplicity" @default.
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- W2055876613 doi "https://doi.org/10.1006/bbrc.2000.3837" @default.
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