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- W2055906857 endingPage "3905" @default.
- W2055906857 startingPage "3887" @default.
- W2055906857 abstract "In the Saccharomyces cerevisiae glycolytic pathway, 11 enzymes catalyze the stepwise conversion of glucose to two molecules of ethanol plus two CO₂ molecules. In the highly crowded cytoplasm, this pathway would be very inefficient if it were dependent on substrate/enzyme diffusion. Therefore, the existence of a multi-enzymatic glycolytic complex has been suggested. This complex probably uses the cytoskeleton to stabilize the interaction of the various enzymes. Here, the role of filamentous actin (F-actin) in stabilization of a putative glycolytic metabolon is reported. Experiments were performed in isolated enzyme/actin mixtures, cytoplasmic extracts and permeabilized yeast cells. Polymerization of actin was promoted using phalloidin or inhibited using cytochalasin D or latrunculin. The polymeric filamentous F-actin, but not the monomeric globular G-actin, stabilized both the interaction of isolated glycolytic pathway enzyme mixtures and the whole fermentation pathway, leading to higher fermentation activity. The associated complexes were resistant against inhibition as a result of viscosity (promoted by the disaccharide trehalose) or inactivation (using specific enzyme antibodies). In S. cerevisiae, a glycolytic metabolon appear to assemble in association with F-actin. In this complex, fermentation activity is enhanced and enzymes are partially protected against inhibition by trehalose or by antibodies." @default.
- W2055906857 created "2016-06-24" @default.
- W2055906857 creator A5001967064 @default.
- W2055906857 creator A5017182512 @default.
- W2055906857 creator A5021806082 @default.
- W2055906857 creator A5028806233 @default.
- W2055906857 creator A5041941918 @default.
- W2055906857 creator A5049085263 @default.
- W2055906857 creator A5078159472 @default.
- W2055906857 date "2013-07-12" @default.
- W2055906857 modified "2023-10-10" @default.
- W2055906857 title "A glycolytic metabolon in<i>Saccharomyces cerevisiae</i>is stabilized by F-actin" @default.
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