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- W2055929559 abstract "The detection of oligonucleotides has been studied using external injection of matrix-assisted laser desorption/ionization (MALDI) produced ions into a quadrupole ion trap storage device. The ions stored in the trap are then detected by pulsed DC ejection into a reflectron time-of-flight mass spectrometer. It is shown that by using a low acceleration voltage from the probe tip and external injection into the trap that 3-mers to 9-mers can be stored for 20–50 ms and detected as intact ions. In comparison, direct MALDI inside the trap resulted in extensive fragmentation as a function of storage time. Using the external injection method with a He buffer gas in the trap yields a resolution of 250–400 in the ion trap storage/reflectron time-of-flight mass spectrometer. The resolution can be further enhanced to 400–800 using a bipolar extraction method. In addition, the trapping efficiency for the injection of externally produced ions into the trap can be enhanced using a dynamic trapping method. The MALDI mass spectra produced by this methodology result in excellent signal-to-noise ratio due to the capabilities of the trap for eliminating low mass matrix background from the spectra. © 1997 John Wiley & Sons, Ltd." @default.
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- W2055929559 date "1997-08-30" @default.
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- W2055929559 title "Detection of Oligonucleotides by External Injection into an Ion Trap Storage/Reflectron Time-of-flight Device" @default.
- W2055929559 doi "https://doi.org/10.1002/(sici)1097-0231(19970830)11:13<1440::aid-rcm25>3.0.co;2-9" @default.
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