Matches in SemOpenAlex for { <https://semopenalex.org/work/W2055936518> ?p ?o ?g. }
Showing items 1 to 49 of
49
with 100 items per page.
- W2055936518 endingPage "329" @default.
- W2055936518 startingPage "329" @default.
- W2055936518 abstract "Sorting and packaging of regulated secretory proteins involves protein aggregation in the trans Golgi network and secretory granules. We characterized the pH-dependent interactions of pancreatic zymogens with Muclin. Muclin is a ~300 kDa sulfated, O-glycosylated protein that is a major component of the mouse zymogen granule. Muclin is derived from pro-Muclin, a type I membrane protein which is proposed to be a Golgi cargo receptor Acini were incubated with [35S]sulfate to label Muclin which was purified to near homogeneity on preparative SDS-PAGE. In solution, [35S]-Muclin co-aggregated with isolated zymogens but did not self-aggregate at mildly acidic pH. In an overlay assay using zymogens immobilized on PVDF membranes, Muclin exhibited direct binding to several zymogens at mildly acidic pH. The Muclin binding partners were identified by N-terminal sequencing and mass spec analysis to be: amylase, pro-lipase, pro-carboxypeptidase A1, pro-elastase II, chymotrypsinogen B, and Reg1. Muclin contains numerous intrachain disulfide bonds in its SRCR and CUB domains which are important for the three dimensional structure of these domains. To test whether the protein conformation is important for Muclin binding to zymogens, Muclin was treated with reducing agents to denature the protein backbone. Reduced Muclin bound as well as untreated Muclin. Pronase digestion of Muclin was used to produce glycopeptides containing only the sulfated, O-glycosylated domains. The glycopeptides bound to zymogens in the overlay assay, and unlabeled glycopeptides competed with binding of labeled intact Muclin. These data demonstrate that the sulfated, O-glycosylated groups are responsible for the pH-dependent interactions of Muclin with the zymogens. The behavior of Muclin fulfils the requirement of a Golgi cargo receptor to bind to regulated secretory proteins under the mildly acidic pH conditions that exist in the trans Golgi network. Supported by NIDDK 55998." @default.
- W2055936518 created "2016-06-24" @default.
- W2055936518 creator A5018850248 @default.
- W2055936518 creator A5038163774 @default.
- W2055936518 date "2004-11-01" @default.
- W2055936518 modified "2023-09-27" @default.
- W2055936518 title "DIRECT BINDING OF THE GOLGI CARGO RECEPTOR MUCLIN TO PANCREATIC ZYMOGENS AT MILDLY ACIDIC pH." @default.
- W2055936518 doi "https://doi.org/10.1097/00006676-200411000-00026" @default.
- W2055936518 hasPublicationYear "2004" @default.
- W2055936518 type Work @default.
- W2055936518 sameAs 2055936518 @default.
- W2055936518 citedByCount "0" @default.
- W2055936518 crossrefType "journal-article" @default.
- W2055936518 hasAuthorship W2055936518A5018850248 @default.
- W2055936518 hasAuthorship W2055936518A5038163774 @default.
- W2055936518 hasBestOaLocation W20559365181 @default.
- W2055936518 hasConcept C108625454 @default.
- W2055936518 hasConcept C119062480 @default.
- W2055936518 hasConcept C155138218 @default.
- W2055936518 hasConcept C158617107 @default.
- W2055936518 hasConcept C185592680 @default.
- W2055936518 hasConcept C55493867 @default.
- W2055936518 hasConceptScore W2055936518C108625454 @default.
- W2055936518 hasConceptScore W2055936518C119062480 @default.
- W2055936518 hasConceptScore W2055936518C155138218 @default.
- W2055936518 hasConceptScore W2055936518C158617107 @default.
- W2055936518 hasConceptScore W2055936518C185592680 @default.
- W2055936518 hasConceptScore W2055936518C55493867 @default.
- W2055936518 hasIssue "4" @default.
- W2055936518 hasLocation W20559365181 @default.
- W2055936518 hasOpenAccess W2055936518 @default.
- W2055936518 hasPrimaryLocation W20559365181 @default.
- W2055936518 hasRelatedWork W108283101 @default.
- W2055936518 hasRelatedWork W1500427933 @default.
- W2055936518 hasRelatedWork W1969817866 @default.
- W2055936518 hasRelatedWork W2033929479 @default.
- W2055936518 hasRelatedWork W2040413583 @default.
- W2055936518 hasRelatedWork W2046000730 @default.
- W2055936518 hasRelatedWork W2063679949 @default.
- W2055936518 hasRelatedWork W2148298538 @default.
- W2055936518 hasRelatedWork W2344835984 @default.
- W2055936518 hasRelatedWork W28284764 @default.
- W2055936518 hasVolume "29" @default.
- W2055936518 isParatext "false" @default.
- W2055936518 isRetracted "false" @default.
- W2055936518 magId "2055936518" @default.
- W2055936518 workType "article" @default.